CAJ | 학술논문

目的：检测肺炎支原体（Mp）感染婴幼儿的细胞免疫功能和体液免疫功能，研究结果的变化规律，探讨临床相关的诊断指标。方法收集肺炎支原体感染的患儿的外周血标本，采用流式细胞术检测淋巴细胞表面分化抗原 CD3、CD4、CD8的表达，利用生化仪检测血清中 IgM、IgG、IgA 的浓度；同时设立健康对照组。结果肺炎支原体患儿外周血 CD3＋ T 淋巴细胞、CD4＋T 淋巴细胞比例、CD4＋／CD8＋淋巴细胞比值和免疫球蛋白 IgA、IgG 浓度低于健康儿童，免疫球蛋白 IgM 浓度高于正常儿童；低拷贝组患儿外周血 CD4＋ T 淋巴细胞比例和免疫球蛋白 IgM 浓度低于高拷贝组。结论肺炎支原体感染的患儿免疫状态发生明显变化，T 淋巴细胞免疫功能被抑制，体液免疫功能发生一定的紊乱。
목적：검측폐염지원체（Mp）감염영유인적세포면역공능화체액면역공능，연구결과적변화규률，탐토림상상관적진단지표。방법수집폐염지원체감염적환인적외주혈표본，채용류식세포술검측림파세포표면분화항원 CD3、CD4、CD8적표체，이용생화의검측혈청중 IgM、IgG、IgA 적농도；동시설립건강대조조。결과폐염지원체환인외주혈 CD3＋ T 림파세포、CD4＋T 림파세포비례、CD4＋／CD8＋림파세포비치화면역구단백 IgA、IgG 농도저우건강인동，면역구단백 IgM 농도고우정상인동；저고패조환인외주혈 CD4＋ T 림파세포비례화면역구단백 IgM 농도저우고고패조。결론폐염지원체감염적환인면역상태발생명현변화，T 림파세포면역공능피억제，체액면역공능발생일정적문란。
Objective To detect the cellular immune and humoral immune function in infants with Mycoplasma pneumoniae(Mp) infection,and to study its change regularity and the related clinical indexes.Methods The peripheral blood samples were collected from infantile patients with MP infection.The expression of CD3,CD4 and CD8 on the surface of T lymphocyte(T-cell)were detec-ted by the flow cytometry and the concentrations of IgG,IgA and IgM in serum were detected by the automatic biochemistry analy-zer.At the same time the normal control group was set up.Results The proportion of CD3 + and CD4 + T-cell,CD4 +/CD8 + ratio and the concentration of IgG,IgA in the patient group were significantly lower than those in the control group,while the concentra-tion of IgM in the patient group were significantly lower than those in the normal children.The concentration of IgM was higher than that in the normal children;the proportion of peripheral blood CD4 + T-cell and the concentration of IgM in the low-copy group were significantly lower than those in the high-copy group.Conclusion The immune status in children with Mp infection has obvi-ous change.The immune function of T-cell is suppressed.the humoral immune function has certain turbulence.