CAJ | 학술논문

目的：探讨胃癌手术前后外周血循环 miR-21表达对胃癌临床病理特征的影响。方法42例原发胃癌手术当日及术后1周抽取外周血，应用实时荧光定量 PCR，以 U6为内参照基因，检测血浆 miR-21相对表达。结果患者胃癌术后血浆 miR-21表达与术前相比明显下调（P ＜0.01），总体相对表达量平均下降了28.2倍；并且发现，有肿瘤家族史的患者术后 miR-21表达上调，而无家族史的胃癌患者术后比术前血浆 miR-21表达平均下降22倍（P ＜0.05）；实验结果还发现，随着患者分化程度由高向低的变化，患者淋巴结转移率逐步上升，同时术后平均 miR-21表达由低向高转变，表现出 miR-21相对表达倍数在高、中、中低和低4个分化区上的分布，分别与淋巴结转移率在4个分化区上的分布呈相关性。结论循环外周血 miR-21检测对胃癌早期辅助诊断及术后恶性程度判断有临床意义。
목적：탐토위암수술전후외주혈순배 miR-21표체대위암림상병리특정적영향。방법42례원발위암수술당일급술후1주추취외주혈，응용실시형광정량 PCR，이 U6위내삼조기인，검측혈장 miR-21상대표체。결과환자위암술후혈장 miR-21표체여술전상비명현하조（P ＜0.01），총체상대표체량평균하강료28.2배；병차발현，유종류가족사적환자술후 miR-21표체상조，이무가족사적위암환자술후비술전혈장 miR-21표체평균하강22배（P ＜0.05）；실험결과환발현，수착환자분화정도유고향저적변화，환자림파결전이솔축보상승，동시술후평균 miR-21표체유저향고전변，표현출 miR-21상대표체배수재고、중、중저화저4개분화구상적분포，분별여림파결전이솔재4개분화구상적분포정상관성。결론순배외주혈 miR-21검측대위암조기보조진단급술후악성정도판단유림상의의。
Objective To investigate effect of peripheral blood miR-21 expression on the clinicopathological features of gastric cancer before and after gastric surgery.Methods The peripheral blood was collected on the operative day and postoperative 7 d in 42 patients with primary gastric cancer surgery.The real-tine quantitative PCR was adopted to detect the relative expression of miR-21 in plasma with U6 as an internal reference gene.Results The plasma miR-21 expression level after gastric cancer operation was significantly down-regulated compared with before operation(P <0.01),the overall relative expression level was dropped by an average of 18.2 times;and it was found that the postoperative miR-21 expression in the patients with the tumor family history was up-regulated,the postoperative plasma miR-21 expression in the patients without the tumor family history was decreased by 22 times compared with before operation(P <0.05);the experimental results also fund that as the patient′s differentiation degree was changed from high to low,the lymph node metastasis rate was gradually increased,while postoperative average miR-21 expression was changed from low to high,showing the relative expression times of miR-21 were distributed in the four differentiated zones of high,middle,middle-low and low,which showed the correlation with the distribution of the lymph node metastasis rate in these four differentiation zones.Conclusion The peripheral blood MiR-21 detection has the clinical significance to early auxiliary diagnosis and the judgement of postoperative malignant degree.