中外医疗
中外醫療
중외의료
CHINA FOREIGN MEDICAL TREATMENT
2014年
25期
32-33
,共2页
血小板减少性紫癜%抗体%血小板糖蛋白
血小闆減少性紫癜%抗體%血小闆糖蛋白
혈소판감소성자전%항체%혈소판당단백
Thrombocytopenic purpura%Antibody%Platelet glycoprotein
目的：探讨特发性血小板减少性紫癜(ITP)患者患病时PAIg的表达评价其在ITP中的临床意义。方法选取40例ITP患儿作为研究对象，同期门诊体检正常儿童20例为对照组，应用酶联免疫吸附法（ELISA法）测定血小板相关抗体(PAIgA、PAIgG)、采取单克隆抗体特异性俘获血小板抗原（MAIPA法）测定血小板特异性抗体抗血小板膜糖蛋白抗体（GPIIb/IIIa、GPIb/IX），全自动血液分析仪测定血小板计数。结果 PAIgA、PAIgG、GPIIb/IIIa、GPIb/IX、血小板计数对照组分别为（4.22±0.78）ng/107PA、（23.17±3.46）ng/107PA、（0.33±0.01）A值、（0.31±0.01）A值、（137.82±42.37）×109/L，ITP组分别为（45.58±5.32） ng/107PA、（243.34±12.21）ng/107PA、（0.42±0.06）A值、（0.41±0.05）A值、（35.50±21.35）×109/L，两组以上各项指标之间比较均差异有统计学意义（P<0.05）；ITP组血小板计数与特异性抗体GPIIb/IIIa、GPIb/IX之间具有负相关，相关系数分别为-0.24（P<0.05）、-0.31（P<0.05）。结论在ITP患儿中血小板相关抗体及特异性抗体均有明显升高，呈现阳性表达，ELISA 法、MAIPA法联合检测有助于鉴别免疫性与非免疫性血小板减少型紫癜，能提高早期诊断率，并且有助于指导临床治疗。
目的：探討特髮性血小闆減少性紫癜(ITP)患者患病時PAIg的錶達評價其在ITP中的臨床意義。方法選取40例ITP患兒作為研究對象，同期門診體檢正常兒童20例為對照組，應用酶聯免疫吸附法（ELISA法）測定血小闆相關抗體(PAIgA、PAIgG)、採取單剋隆抗體特異性俘穫血小闆抗原（MAIPA法）測定血小闆特異性抗體抗血小闆膜糖蛋白抗體（GPIIb/IIIa、GPIb/IX），全自動血液分析儀測定血小闆計數。結果 PAIgA、PAIgG、GPIIb/IIIa、GPIb/IX、血小闆計數對照組分彆為（4.22±0.78）ng/107PA、（23.17±3.46）ng/107PA、（0.33±0.01）A值、（0.31±0.01）A值、（137.82±42.37）×109/L，ITP組分彆為（45.58±5.32） ng/107PA、（243.34±12.21）ng/107PA、（0.42±0.06）A值、（0.41±0.05）A值、（35.50±21.35）×109/L，兩組以上各項指標之間比較均差異有統計學意義（P<0.05）；ITP組血小闆計數與特異性抗體GPIIb/IIIa、GPIb/IX之間具有負相關，相關繫數分彆為-0.24（P<0.05）、-0.31（P<0.05）。結論在ITP患兒中血小闆相關抗體及特異性抗體均有明顯升高，呈現暘性錶達，ELISA 法、MAIPA法聯閤檢測有助于鑒彆免疫性與非免疫性血小闆減少型紫癜，能提高早期診斷率，併且有助于指導臨床治療。
목적：탐토특발성혈소판감소성자전(ITP)환자환병시PAIg적표체평개기재ITP중적림상의의。방법선취40례ITP환인작위연구대상，동기문진체검정상인동20례위대조조，응용매련면역흡부법（ELISA법）측정혈소판상관항체(PAIgA、PAIgG)、채취단극륭항체특이성부획혈소판항원（MAIPA법）측정혈소판특이성항체항혈소판막당단백항체（GPIIb/IIIa、GPIb/IX），전자동혈액분석의측정혈소판계수。결과 PAIgA、PAIgG、GPIIb/IIIa、GPIb/IX、혈소판계수대조조분별위（4.22±0.78）ng/107PA、（23.17±3.46）ng/107PA、（0.33±0.01）A치、（0.31±0.01）A치、（137.82±42.37）×109/L，ITP조분별위（45.58±5.32） ng/107PA、（243.34±12.21）ng/107PA、（0.42±0.06）A치、（0.41±0.05）A치、（35.50±21.35）×109/L，량조이상각항지표지간비교균차이유통계학의의（P<0.05）；ITP조혈소판계수여특이성항체GPIIb/IIIa、GPIb/IX지간구유부상관，상관계수분별위-0.24（P<0.05）、-0.31（P<0.05）。결론재ITP환인중혈소판상관항체급특이성항체균유명현승고，정현양성표체，ELISA 법、MAIPA법연합검측유조우감별면역성여비면역성혈소판감소형자전，능제고조기진단솔，병차유조우지도림상치료。
Objective To understand the expression of PAIg in patients with idiopathic thrombocytopenic purpura (ITP) and evalu-ate its clinical significance in ITP. Methods 40 children with ITP were selected as the subjects, and 20 normal children underwent outpatient physical examination during the same period were selected as the control group. Enzyme linked immunosorbent assay (ELISA) was used to detect platelet-associated antibodies (PAIgA, PAIgG), monoclonal antibody immobilization of platelet antigen assay (MAIPA) was adopted to determine platelet specific antibodies against platelet membrane glycoprotein antibody (GPIIb/IIIa, GPIb/IX), and full automatic blood analyzer was used to determine the platelet count. Results The PAIgA, PAIgG, GPIIb/IIIa, GPIb/IX and platelet count respectively was (4.22 ±0.78)ng/107PA, (23.17 ±3.46)ng/107PA, (0.33 ±0.01)A, (0.31 ±0.01)A, (137.82 ± 42.37) ×109/L in the control group, and respectively was (45.58 ±5.32)ng/107PA, (243.34 ±12.21)ng/107PA, (0.42 ±0.06)A, (0.41 ± 0.05)A, (35.50±21.35)×109/L in the control group, the differences between the two groups were statistically significant(P<0.05); the platelet count of the ITP group was negatively correlated with the specific antibodies GPIIb/IIIa and GPIb/IX, the correlation coef-ficient was -0.24(P<0.05), -0.31(P<0.05), respectively. Conclusion Platelet associated antibodies and specific antibodies increase significantly in children with ITP, and show positive expression; ELISA combined with MAIPA is help to differentiate immune thrombocytopenia purpura and non-immune thrombocytopenia purpura, which can improve the early diagnosis rate, and is con-ducive to guiding the clinical treatment.
