CAJ | 학술논문

目的：检测CD56、CD54在甲状腺病变组织中的表达情况，探讨CD56、CD54的表达在甲状腺病变鉴别诊断中的作用。方法分别收集海南医学院附属医院病理科73例甲状腺乳头状癌、64例良性病变组织蜡块。将137例石蜡标本制做组织芯片。应用免疫组织化学技术检测CD56、CD54蛋白在甲状腺病变组织中的表达情况。结果 CD56在甲状腺乳头状癌组织中大多表达缺失，表达率为14%，在良性甲状腺病变组织中表达率高(92.2%)，两者间表达率差异具有显著统计学意义(P<0.01)。CD54在甲状腺乳头状癌及桥本氏甲状腺炎组织中表达增高(69.9%vs 75.0%)，呈弥漫阳性表达，这两组间表达差异无统计学意义(P>0.05)；在其他良性甲状腺组织包括结节性甲状腺肿和滤泡性腺瘤中，CD54表达较低(3.8%)，主要呈散在弱阳性表达，与甲状腺乳头状癌组织相比，其表达差异具有显著统计学意义(P<0.01)。结论 CD56在甲状腺乳头状癌中表达缺失，在甲状腺良性病变组织中表达增高；CD54在甲状腺乳头状癌及桥本氏甲状腺炎中高表达，联合检测CD56、CD54对甲状腺病变的鉴别诊断具有重要作用。
목적：검측CD56、CD54재갑상선병변조직중적표체정황，탐토CD56、CD54적표체재갑상선병변감별진단중적작용。방법분별수집해남의학원부속의원병이과73례갑상선유두상암、64례량성병변조직사괴。장137례석사표본제주조직심편。응용면역조직화학기술검측CD56、CD54단백재갑상선병변조직중적표체정황。결과 CD56재갑상선유두상암조직중대다표체결실，표체솔위14%，재량성갑상선병변조직중표체솔고(92.2%)，량자간표체솔차이구유현저통계학의의(P<0.01)。CD54재갑상선유두상암급교본씨갑상선염조직중표체증고(69.9%vs 75.0%)，정미만양성표체，저량조간표체차이무통계학의의(P>0.05)；재기타량성갑상선조직포괄결절성갑상선종화려포성선류중，CD54표체교저(3.8%)，주요정산재약양성표체，여갑상선유두상암조직상비，기표체차이구유현저통계학의의(P<0.01)。결론 CD56재갑상선유두상암중표체결실，재갑상선량성병변조직중표체증고；CD54재갑상선유두상암급교본씨갑상선염중고표체，연합검측CD56、CD54대갑상선병변적감별진단구유중요작용。
Objective To detect the expression levels of CD56 and CD54 in different thyroid lesions, and dis-cuss the application of CD56 and CD54 in the differential diagnosis of thyroid lesions. Methods Paraffin samples in-cluding 73 cases of papillary thyroid carcinoma (PTC) and 64 cases of thyroid benign hyperplasia tissues from pa-thology department of Hainan medical college affiliated hospital were collected to be made as tissue chips. And then immunohistochemical techniques were applied to detect the expression levels of CD56 and CD54 in thyroid lesions. Results The expression level of CD56 in benign tissue was higher than that in PTC (92.2%vs 14%, P<0.01). Com-pared with the groups of nodular goiter and follicular adenoma, the CD56 positive expression rate in PTC was higher with a significant difference (69.9% vs 3.8%, P<0.01). However, there was no difference in the positive expression rate of CD56 between PTC and Hashimoto's thyroiditis (69.9%and 75.0%, P>0.05). Conclusion With lower expres-sion of CD56 in PTC rather than in benign tumor and higher expression of CD54 in PTC and Hashimoto's thyroiditis, the combined detection of CD56 and CD54 might contribute to differential diagnosis of the thyroid diseases.