CAJ | 학술논문

目的：分析磷酸化的血管内皮生长因子受体3(P-VEGFR-3)、磷酸化的蛋白激酶(P-AKT)、磷酸化的血小板源性生长因子α受体(P-PDGFR-α)及淋巴内皮标记物D2-40在子宫内膜癌、子宫内膜不典型增生及子宫内膜炎症性疾病中的蛋白表达差异，以探讨其临床检测价值。方法对有淋巴结转移的子宫内膜癌、无淋巴结转移的子宫内膜癌、子宫内膜不典型增生及子宫内膜炎症性疾病患者的石蜡组织标本各25例进行免疫组织化学染色，显微镜下观察P-VEGFR-3、P-AKT、P-PDGFR-α及D2-40的阳性表达情况。应用SPSS16.0统计学软件分析各组之间的差异性，等级资料和率的比较分别采用Riddit分析及Fisher检验。结果有淋巴结转移的子宫内膜癌组织P-VEGFR-3、P-AKT、P-PDGFR-α及D2-40的阳性表达率和阳性表达程度均明显高于无淋巴转移的子宫内膜癌组织，且无淋巴转移的子宫内膜癌组织P-VEGFR-3、P-AKT、P-PDGFR-α及D2-40的阳性表达率和阳性表达程度均明显高于子宫内膜不典型增生组织，各指标的组间比较差异均有统计学意义(P<0.05)，但子宫内膜不典型增生和炎症性疾病组织之间各指标比较差异均无统计学意义(P>0.05)。结论 P-VEGFR-3、P-AKT、P-PDGFR-α及D2-40与子宫内膜癌的发生及淋巴结转移密切相关，临床上可作为其发病和预后的评估指标。
목적：분석린산화적혈관내피생장인자수체3(P-VEGFR-3)、린산화적단백격매(P-AKT)、린산화적혈소판원성생장인자α수체(P-PDGFR-α)급림파내피표기물D2-40재자궁내막암、자궁내막불전형증생급자궁내막염증성질병중적단백표체차이，이탐토기림상검측개치。방법대유림파결전이적자궁내막암、무림파결전이적자궁내막암、자궁내막불전형증생급자궁내막염증성질병환자적석사조직표본각25례진행면역조직화학염색，현미경하관찰P-VEGFR-3、P-AKT、P-PDGFR-α급D2-40적양성표체정황。응용SPSS16.0통계학연건분석각조지간적차이성，등급자료화솔적비교분별채용Riddit분석급Fisher검험。결과유림파결전이적자궁내막암조직P-VEGFR-3、P-AKT、P-PDGFR-α급D2-40적양성표체솔화양성표체정도균명현고우무림파전이적자궁내막암조직，차무림파전이적자궁내막암조직P-VEGFR-3、P-AKT、P-PDGFR-α급D2-40적양성표체솔화양성표체정도균명현고우자궁내막불전형증생조직，각지표적조간비교차이균유통계학의의(P<0.05)，단자궁내막불전형증생화염증성질병조직지간각지표비교차이균무통계학의의(P>0.05)。결론 P-VEGFR-3、P-AKT、P-PDGFR-α급D2-40여자궁내막암적발생급림파결전이밀절상관，림상상가작위기발병화예후적평고지표。
Objective To analyze the protein expression difference of P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 in the pathological tissues of endometrial cancer, atypical hyperplasia of endometrium (AHE) and endome-trial inflammatory disease (EID), so as to explore their clinical detection value. Methods The pathological tissues of endometrial cancer with lymphatic metastasis, endometrial cancer without lymphatic metastasis, AHE and EID (each with 25 samples) were observed under the microscope after immunohistochemistry, and the positive expression of P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 were detected and analyzed with Riddit, Fisher and SPSS16.0 statisti-cal software. Results The positive expression rate and degree of P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 in endometrial cancer with lymphatic metastasis were all higher than those in endometrial cancer without lymphatic me-tastasis;the positive expression rate and degree of P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 in endometrial can-cer without lymphatic metastasis were all higher than those in AHE;and the difference had statistical significance (P<0.05). But there was no statistically significant difference in positive expression rate and degree of P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 between AHE and EID (P>0.05). Conclusion P-VEGFR-3, P-AKT, P-PDGFR-αand D2-40 are closely related to the occurrence of endometrial cancer and lymphatic metastasis, thus could provide evaluation index for clinical diagnosis and prognosis evaluation.