海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2014年
24期
3592-3595
,共4页
血管紧张素Ⅱ%氯沙坦%足细胞%葡萄糖转运蛋白4
血管緊張素Ⅱ%氯沙坦%足細胞%葡萄糖轉運蛋白4
혈관긴장소Ⅱ%록사탄%족세포%포도당전운단백4
AngiotensinⅡ%Losartan%Podocyte%Glucose transporter 4
目的：观察血管紧张素Ⅱ(AngiotensinⅡ，AngⅡ)对足细胞葡萄糖转运蛋白4(GLUT4)表达的影响，及血管紧张素1型受体阻断剂氯沙坦(Losartan，Lo)能否抑制AngII对足细胞GLUT4的作用。方法将小鼠MPC5足细胞分成对照组、AngⅡ10-6 mmol/L组、AngⅡ10-8 mmol/L组、AngⅡ10-10 mmol/L组、Lo10-6 mmol/L+AngⅡ10-6 mmol/L组，半定量聚合酶链反应(RT-PCR)检测足细胞GLUT4mRNA的水平，间接免疫荧光检测GLUT4蛋白的表达。结果与对照组相比，AngⅡ10-6 mmol/L组能够显著抑制GLUT4的表达及蛋白合成，GLUT4 mRNA的表达下降了56.1%(P=0.041)，间接免疫荧光表达下降了54.9%(P<0.05)。AngⅡ10-8 mmol/L组及AngⅡ10-10 mmol/L组GLUT4的表达与对照组相比有所下降，但差异无统计学意义(P>0.05)，AngⅡ10-8 mmol/L组对GLUT4抑制强于AngⅡ10-10 mmol/L组，两组间差异无统计学意义(P>0.05)。Lo 10-6 mmol/L+AngⅡ10-6 mmol/L组GLUT4的表达显著升高，与AngⅡ10-6组相比，mRNA升高176.3%(P=0.006)，蛋白表达升高87.8%(P<0.05)。结论 AngⅡ能够显著抑制足细胞GLUT4的表达及合成，具有浓度依赖性，这种作用能被氯沙坦所阻断。
目的：觀察血管緊張素Ⅱ(AngiotensinⅡ，AngⅡ)對足細胞葡萄糖轉運蛋白4(GLUT4)錶達的影響，及血管緊張素1型受體阻斷劑氯沙坦(Losartan，Lo)能否抑製AngII對足細胞GLUT4的作用。方法將小鼠MPC5足細胞分成對照組、AngⅡ10-6 mmol/L組、AngⅡ10-8 mmol/L組、AngⅡ10-10 mmol/L組、Lo10-6 mmol/L+AngⅡ10-6 mmol/L組，半定量聚閤酶鏈反應(RT-PCR)檢測足細胞GLUT4mRNA的水平，間接免疫熒光檢測GLUT4蛋白的錶達。結果與對照組相比，AngⅡ10-6 mmol/L組能夠顯著抑製GLUT4的錶達及蛋白閤成，GLUT4 mRNA的錶達下降瞭56.1%(P=0.041)，間接免疫熒光錶達下降瞭54.9%(P<0.05)。AngⅡ10-8 mmol/L組及AngⅡ10-10 mmol/L組GLUT4的錶達與對照組相比有所下降，但差異無統計學意義(P>0.05)，AngⅡ10-8 mmol/L組對GLUT4抑製彊于AngⅡ10-10 mmol/L組，兩組間差異無統計學意義(P>0.05)。Lo 10-6 mmol/L+AngⅡ10-6 mmol/L組GLUT4的錶達顯著升高，與AngⅡ10-6組相比，mRNA升高176.3%(P=0.006)，蛋白錶達升高87.8%(P<0.05)。結論 AngⅡ能夠顯著抑製足細胞GLUT4的錶達及閤成，具有濃度依賴性，這種作用能被氯沙坦所阻斷。
목적：관찰혈관긴장소Ⅱ(AngiotensinⅡ，AngⅡ)대족세포포도당전운단백4(GLUT4)표체적영향，급혈관긴장소1형수체조단제록사탄(Losartan，Lo)능부억제AngII대족세포GLUT4적작용。방법장소서MPC5족세포분성대조조、AngⅡ10-6 mmol/L조、AngⅡ10-8 mmol/L조、AngⅡ10-10 mmol/L조、Lo10-6 mmol/L+AngⅡ10-6 mmol/L조，반정량취합매련반응(RT-PCR)검측족세포GLUT4mRNA적수평，간접면역형광검측GLUT4단백적표체。결과여대조조상비，AngⅡ10-6 mmol/L조능구현저억제GLUT4적표체급단백합성，GLUT4 mRNA적표체하강료56.1%(P=0.041)，간접면역형광표체하강료54.9%(P<0.05)。AngⅡ10-8 mmol/L조급AngⅡ10-10 mmol/L조GLUT4적표체여대조조상비유소하강，단차이무통계학의의(P>0.05)，AngⅡ10-8 mmol/L조대GLUT4억제강우AngⅡ10-10 mmol/L조，량조간차이무통계학의의(P>0.05)。Lo 10-6 mmol/L+AngⅡ10-6 mmol/L조GLUT4적표체현저승고，여AngⅡ10-6조상비，mRNA승고176.3%(P=0.006)，단백표체승고87.8%(P<0.05)。결론 AngⅡ능구현저억제족세포GLUT4적표체급합성，구유농도의뢰성，저충작용능피록사탄소조단。
Objective To investigate the influence of angiotensinⅡ(AngⅡ) on the expression of GLUT4 and whether losartan (Lo) could depress the function of AngⅡon podocytes. Methods The mouse podocyte clone 5 (MPC5) were divided into five groups: the control group, AngⅡ at the concentration of 10-6, 10-8, 10-10 mmol/L groups, and Lo 10-6 mmol/L+AngⅡ10-6 mmol/L group. The GLUT4 mRNA level was detected by semi-quantitative RT-PCR and the expression of GLUT4 by indirect immunofluorescence. Results Compared to the control group, in group AngⅡ10-6 mmol/L, the GLUT4 expression and protein synthesis were significantly decreased, GLUT4 mRNA transcription was decreased by 56.1%(P=0.041), and indirect immunofluorescence expression was decreased by 54.9%. The GLUT4 expression of AngⅡ10-10 mmol/L and AngⅡ10-8 mmol/L group was decreased compared with the control group, but there were no statistically significant difference. AngⅡ10-8 mmol/L depressed more GLUT4 expression than the AngⅡ10-10 mmol/L did, and there were also no statistically significant difference (P>0.05). As for the Lo 10-6 mmol/L+AngⅡ10-6 mmol/L group, the GLUT4 expression was significantly increased, when compared with the AngⅡ10-6 group its GLUT4 mRNA transcription was increased by 176.3%(P=0.006), and indirect immunofluorescence expres-sion increased by 87.8%(P<0.05). Conclusion AngⅡcould significantly decrease the GLUT4 expression and synthe-sis, and the effect was concentration dependent therefore could be hampered by Losartan.
