CAJ | 학술논문

目的：研究α-硫辛酸(Alpha-lipoic acid，ALA)对实验性变态反应性脑脊髓炎(Experimental autoimmune encephalomyelitis，EAE)大鼠模型中脊髓组织IL-17表达的影响。方法健康雌性Wistar大鼠50只，随机分为正常对照组(NC组)，EAE 7 d组(EAE-7 d组)、EAE 6个月病程组(EAE-6 m组)、硫辛酸急性期组(ALA-7 d组)和硫辛酸6个月病程组(ALA-6 m组)。HE染色观察病理学改变，免疫组织化学染色观察IL-17的表达变化。结果 HE染色结果显示，在α-硫辛酸治疗急性期组和6个月病程组，均可减轻脊髓组织的炎性反应和髓鞘损伤。免疫细胞化学染色结果显示，EAE-7 d组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01)；EAE-6 m组大鼠IL-17蛋白的表达比正常对照组增加(P<0.01)；α-硫辛酸处理后急性期组和6个月病程组IL-17蛋白的表达明显降低(P<0.01)。结论α-硫辛酸在EAE大鼠模型中的保护作用可能是通过抑制IL-17的表达来实现的。
목적：연구α-류신산(Alpha-lipoic acid，ALA)대실험성변태반응성뇌척수염(Experimental autoimmune encephalomyelitis，EAE)대서모형중척수조직IL-17표체적영향。방법건강자성Wistar대서50지，수궤분위정상대조조(NC조)，EAE 7 d조(EAE-7 d조)、EAE 6개월병정조(EAE-6 m조)、류신산급성기조(ALA-7 d조)화류신산6개월병정조(ALA-6 m조)。HE염색관찰병이학개변，면역조직화학염색관찰IL-17적표체변화。결과 HE염색결과현시，재α-류신산치료급성기조화6개월병정조，균가감경척수조직적염성반응화수초손상。면역세포화학염색결과현시，EAE-7 d조대서IL-17단백적표체비정상대조조증가(P<0.01)；EAE-6 m조대서IL-17단백적표체비정상대조조증가(P<0.01)；α-류신산처리후급성기조화6개월병정조IL-17단백적표체명현강저(P<0.01)。결론α-류신산재EAE대서모형중적보호작용가능시통과억제IL-17적표체래실현적。
Objective To explore the effects of alpha-lipoic acid (ALA) on IL-17 expression in rats with ex-perimental allergic encephalomyelitis (EAE). Methods Fifty female Wistar rats were divided randomly into five groups: the normal control group (NC group), EAE model group with 7 days (EAE-7 d group), EAE model group with 6 months (EAE-6 m group), ALA administrated EAE model group with 7 days (ALA treated EAE-7 d group), ALA administrated EAE group with 6 months (ALA treated EAE-6 m group). HE staining was used to detect the pathologic changes. Immunohistochemistry was used to detect the expression of IL-17. Results HE staining showed that, in alpha-lipoic acid treated groups (ALA treated EAE-7 d group, ALA treated EAE-6 m group), inflammatory reac-tion and myelin damage were all relieved. According to immunohistochemistry, the expression of IL-17 in EAE-7 d group and in EAE-6 m group was significantly increased compared to NC group (P<0.01). However, the expression of IL-17 in alpha-lipoic acid treated groups significantly decreased compared with EAE-7 d group and EAE-6 m group re-spectively (P<0.01). Conclusion Alpha-lipoic acid may exert its neuroprotective effect through ameliorating inflamma-tive reacts.