CAJ | 학술논문

目的：比较实时 PCR 法和 PCR-反向点杂交法（PCR-RDB）检测 HPV 的一致性。方法收集109份女性生殖道样本，利用实时 PCR 法和 PCR-RDB 法分别检测 HPV 感染和基因型分布情况，不一致样本采用 PCR-悬浮芯片杂交法复检。结果83.5％（91／109）的样本两种方法结果一致（kappa＝0.671），18例不一致样本 PCR-悬浮芯片杂交法复检显示7例与实时 PCR相符，11例与 PCR-RDB 相符；高、低病毒载量组间 PCR-RDB 法的检测结果差异无统计学意义（χ2＝1.476，P ＝0.224）。结论实时 PCR 和 PCR-RDB 两法用于 HPV 检测一致性一般；HPV 病毒载量在103～108范围内时 PCR-RDB 法的阳性率较稳定。
목적：비교실시 PCR 법화 PCR-반향점잡교법（PCR-RDB）검측 HPV 적일치성。방법수집109빈녀성생식도양본，이용실시 PCR 법화 PCR-RDB 법분별검측 HPV 감염화기인형분포정황，불일치양본채용 PCR-현부심편잡교법복검。결과83.5％（91／109）적양본량충방법결과일치（kappa＝0.671），18례불일치양본 PCR-현부심편잡교법복검현시7례여실시 PCR상부，11례여 PCR-RDB 상부；고、저병독재량조간 PCR-RDB 법적검측결과차이무통계학의의（χ2＝1.476，P ＝0.224）。결론실시 PCR 화 PCR-RDB 량법용우 HPV 검측일치성일반；HPV 병독재량재103～108범위내시 PCR-RDB 법적양성솔교은정。
Objective To compare real time PCR with PCR-reverse dot blot hybridization (PCR-RDB)for detecting human pap-illomavirus (HPV)infection in women.Methods A total of 109 genital specimens from women were collected in the study.All specimens were tested HPV by using real time PCR and PCR-RDB,discrepant samples were tested again by PCR-xMAP.Results The concordant rate was 83.5%(91/109)between real time PCR and PCR-RDB (kappa=0.671),the other 18 discrepant samples were retested by PCR-xMAP,7 of those were identical with real time PCR and 11 with PCR-RDB.No differences of PCR-RDB pos-itive rates were found between the high and low viral load groups (χ2 =1.476,P =0.224).Conclusion It demonstrated moderate consistency between real time PCR and PCR-RDB.The HPV positive rates of PCR-RDB were stable when the viral loads were 103-108 .