CAJ | 학술논문

目的：探讨血管紧张素原（AGT ）基因 T174M、M235T 多态性及血糖水平与动脉硬化性脑梗死的关系。方法采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法检测 AGT 基因 T174M、M235T 位点基因型的多态性，用全自动生化分析仪测定血糖等生化指标。结果 AGT 基因 T174M 位点携带 TT 基因型者、TM 基因型者 ACI 组 GLU 水平高于健康对照组有统计学差异（P ＜0.05），两组不同基因型间血糖水平比较差异无统计学意义（P ＞0.05）；M235T 位点 MM、MT、TT 基因型者ACI 组 GLU 水平高于健康对照组比较差异有统计学意义（P ＜0.05），两组不同基因型间血糖水平比较差异无统计学意义（P ＞0.05）。结论血管紧张素原（AGT）基因 T174M、M235T 多态性及血糖水平与动脉硬化性脑梗死三者之间未发现关联性；高血糖是动脉硬化性脑梗死的发生的危险因素之一。
목적：탐토혈관긴장소원（AGT ）기인 T174M、M235T 다태성급혈당수평여동맥경화성뇌경사적관계。방법채용취합매련식반응-한제성편단장도다태성（PCR-RFLP）방법검측 AGT 기인 T174M、M235T 위점기인형적다태성，용전자동생화분석의측정혈당등생화지표。결과 AGT 기인 T174M 위점휴대 TT 기인형자、TM 기인형자 ACI 조 GLU 수평고우건강대조조유통계학차이（P ＜0.05），량조불동기인형간혈당수평비교차이무통계학의의（P ＞0.05）；M235T 위점 MM、MT、TT 기인형자ACI 조 GLU 수평고우건강대조조비교차이유통계학의의（P ＜0.05），량조불동기인형간혈당수평비교차이무통계학의의（P ＞0.05）。결론혈관긴장소원（AGT）기인 T174M、M235T 다태성급혈당수평여동맥경화성뇌경사삼자지간미발현관련성；고혈당시동맥경화성뇌경사적발생적위험인소지일。
Objective To study the relationship among the angiotensinogen (AGT)gene T174M,M235T polymorphisma,blood glucose level and atherosclerotic cerebral infarction.Methods The polymerase chain reaction-restriction fragment length polymor-phism (PCR-RFLP)method was adopted to detecte the gene polymorphisms of AGT gene 174,235 sites and the fully automatic bi-ochemical analyzer was used to detect the biochemical indexes of GLU,etc.in 396 patients with atherosclerotic cerebral infarction and 360 normal controls.Results The GLU level in the patients of the ACI group carrying genotype TT and TM at AGT gene T174M site was higher than that in the normal control group with statistical differences(P <0.05),the glucose level had no statisti-cal difference between the different 2 kinds of genotype (P >0.05);the glucose level in the patients carrying genotypes MM,MT and TT at M235T site in the ACI group was higher than that in the normal group,and the difference was statistically significant(P<0.05).The blood glucose level between the two groups carrying 2 different kinds of genotype showed no statistically significant difference (P >0.05 ).Conclusion No correlation is found among AGT gene T174M,M235T polymorphism,blood glucose level and atherosclerotic cerebral infarction;hyperglycemia is one of the risk factors of atherosclerotic cerebral infarction occurrence.