CAJ | 학술논문

目的：探讨异硫氰酸荧光素(FITC）标记阳离子脂质体通过尾静脉注入后，跨越大鼠血脊髓屏障并在局部聚集的情况。方法30只成年Wistar大鼠随机等分为3组，每组10只，荧光显微镜动态观察脂质体在中枢神经系统(CNS）中靶向聚集与扩散情况，通过组织学观测进一步分析其与CNS细胞的关系。结果相同浓度下(75~600μg/mL），TAT-PEG-阳离子脂质体较未经TAT修饰者具更低细胞毒性，且更易被MCF-7细胞摄取；FITC标记TAT-PEG-阳离子脂质体可跨越BSCB并聚集，低倍镜下观察到许多荧光微粒聚集于CNS组织，高倍镜形象地显示出荧光微粒位于神经细胞内。结论经跨膜肽(TAT）、聚乙二醇(PEG）修饰的阳离子脂质体具有良好的生物相容性与靶向定位特性，可跨过BSCB，聚集于CNS细胞中，安全用作药物载体，为中枢神经系统疾病的治疗提供新的途径。
목적：탐토이류청산형광소(FITC）표기양리자지질체통과미정맥주입후，과월대서혈척수병장병재국부취집적정황。방법30지성년Wistar대서수궤등분위3조，매조10지，형광현미경동태관찰지질체재중추신경계통(CNS）중파향취집여확산정황，통과조직학관측진일보분석기여CNS세포적관계。결과상동농도하(75~600μg/mL），TAT-PEG-양리자지질체교미경TAT수식자구경저세포독성，차경역피MCF-7세포섭취；FITC표기TAT-PEG-양리자지질체가과월BSCB병취집，저배경하관찰도허다형광미립취집우CNS조직，고배경형상지현시출형광미립위우신경세포내。결론경과막태(TAT）、취을이순(PEG）수식적양리자지질체구유량호적생물상용성여파향정위특성，가과과BSCB，취집우CNS세포중，안전용작약물재체，위중추신경계통질병적치료제공신적도경。
Objective?To?observe?the?ability?of?fluorescein?isothiocyanate(FITC)?loaded?transactivating-transduction?protein(TAT)-polyethylene?glycol(PEG)-modified?liposomes?crossing?the?blood-spinal?cord?barrier(BSCB)?of?rats,?then?localizing?there?after?injection?of?caudal?veins.?Method?Thirty?rats?were?divided?into?three?groups.?Rats?were?injected?with?liposome?solution?(4.55?mg/kg)?via?the?caudal?vein.?Using?fluorescence?microscope?and?histological?methods?to?analyse.?Results?At?the?same?concentration(75-600?μg/mL),?TAT-modified?liposomes?had?lower?cytotoxicity,?and?were?more?easily?uptaken?by?MCF-7?cells?than?the?ones?without?TAT.?We?observed?that?TAT-modified?liposomes?crossed?the?BSCB,?accumulated?in?CNS?tissue?and?aggregated?into?cells?there?by?microscope.?Conclusion?TAT-PEG-modified?liposomes?have?the?characteristics?of?bio-compatibility?and?targeting.?They?can?cross?the?BSCB?acting?as?a?drug?carrier?and?accumulate?in?CNS?tissue?for?future?CNS?disease?treatment.