光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2015年
1期
189-192
,共4页
丛妍斌%郑永红%郑莉%吴飞%谭克俊
叢妍斌%鄭永紅%鄭莉%吳飛%譚剋俊
총연빈%정영홍%정리%오비%담극준
全氟辛烷磺酸%金纳米粒子%比色法%紫外/可见光谱
全氟辛烷磺痠%金納米粒子%比色法%紫外/可見光譜
전불신완광산%금납미입자%비색법%자외/가견광보
Perfluorooctanesulfonate (PFOS)%Gold nanoparticles%Colorimetric method%UV/Vis spectroscopy
全氟辛烷磺酸（perfluorooctanesulfonate ，PFOS ）具有遗传毒性、生物蓄积性和持久性，且难以降解，因此对其进行分析研究具有十分重要的意义。实验发现，PFOS能使巯基乙胺包被的正电金纳米粒子发生聚集，引起体系吸收信号及颜色改变，据此建立了检测PFOS的紫外-可见分光光度法及比色法。线性方程为 A＝－0.346＋0.049c ，相关系数为0.9924，线性范围0.8～8.0μmol?L －1，检出限为80 nmol?L －1。研究表明：金纳米粒子在524 nm有特征吸收峰，在650 nm处有较宽吸收峰，PFOS的加入会使金纳米粒子524 nm吸收峰降低，650 nm吸收峰增强，随着PFOS浓度增大，体系颜色由酒红色向红紫色变化。表征了体系的扫描电镜显微成像（SEM ）及紫外吸收光谱，考察了金纳米粒子的聚集情况，实验缓冲体系选用pH 5.0的 HAc-NaAc缓冲溶液。本方法具有简单、快速等特点，可通过肉眼观察颜色变化来实现对环境污染物PFOS的检测。本方法用于实际水样中PFOS的检测，RSD≤4.4％。
全氟辛烷磺痠（perfluorooctanesulfonate ，PFOS ）具有遺傳毒性、生物蓄積性和持久性，且難以降解，因此對其進行分析研究具有十分重要的意義。實驗髮現，PFOS能使巰基乙胺包被的正電金納米粒子髮生聚集，引起體繫吸收信號及顏色改變，據此建立瞭檢測PFOS的紫外-可見分光光度法及比色法。線性方程為 A＝－0.346＋0.049c ，相關繫數為0.9924，線性範圍0.8～8.0μmol?L －1，檢齣限為80 nmol?L －1。研究錶明：金納米粒子在524 nm有特徵吸收峰，在650 nm處有較寬吸收峰，PFOS的加入會使金納米粒子524 nm吸收峰降低，650 nm吸收峰增彊，隨著PFOS濃度增大，體繫顏色由酒紅色嚮紅紫色變化。錶徵瞭體繫的掃描電鏡顯微成像（SEM ）及紫外吸收光譜，攷察瞭金納米粒子的聚集情況，實驗緩遲體繫選用pH 5.0的 HAc-NaAc緩遲溶液。本方法具有簡單、快速等特點，可通過肉眼觀察顏色變化來實現對環境汙染物PFOS的檢測。本方法用于實際水樣中PFOS的檢測，RSD≤4.4％。
전불신완광산（perfluorooctanesulfonate ，PFOS ）구유유전독성、생물축적성화지구성，차난이강해，인차대기진행분석연구구유십분중요적의의。실험발현，PFOS능사구기을알포피적정전금납미입자발생취집，인기체계흡수신호급안색개변，거차건립료검측PFOS적자외-가견분광광도법급비색법。선성방정위 A＝－0.346＋0.049c ，상관계수위0.9924，선성범위0.8～8.0μmol?L －1，검출한위80 nmol?L －1。연구표명：금납미입자재524 nm유특정흡수봉，재650 nm처유교관흡수봉，PFOS적가입회사금납미입자524 nm흡수봉강저，650 nm흡수봉증강，수착PFOS농도증대，체계안색유주홍색향홍자색변화。표정료체계적소묘전경현미성상（SEM ）급자외흡수광보，고찰료금납미입자적취집정황，실험완충체계선용pH 5.0적 HAc-NaAc완충용액。본방법구유간단、쾌속등특점，가통과육안관찰안색변화래실현대배경오염물PFOS적검측。본방법용우실제수양중PFOS적검측，RSD≤4.4％。
For the property of persistent ,bioaccumulation and genetic toxicity ,perfluorooctanesulfonate (PFOS) is classified as a sort of persistent organic pollutants (POPs) .It is significant to develop a novel assay for the determination of PFOS .In this work ,we create a new colorimetric assay for PFOS in which the positively-charged gold nanoparticles (AuNPs) work as a nano-probe .This method works on the aggregation of AuNPs induced by PFOS via electrostatic interaction .The stable monodisperse AuNPs coated by cysteamine present color of red wine and the addition of PFOS can make the monodispersed AuNPs aggregated resulting in the color change from wine red to reddish purple with a red-shift in ultraviolet-visible absorption spectrum .The ex-perimental results show that AuNPs has a characteristic absorption peak (524 nm) ,as well as a wide absorption peak (650 nm) and the absorption signal intensity is proportional to the PFOS content in a range of 0.8~8.0 μmol?L -1 .According to these , we developed a method based on ultraviolet-visible absorption and colorimetric to detect PFOS with the detection limit of 80 nmol?L -1 .The scanning electron microscope (SEM ) was investigated and the photos show that the stable AuNPs are made and the degree of AuNPs aggregation is related with PFOS concentration .The effect tests of coexisting substances in system show that common anions had less impact on the system and inorganic metal ions had some interference ,which can be get rid of by cat-ion exchange resin in real sample .This assay was applied to detect PFOS in tap water with a recovery range of 87.5% ~118%and RSD≤4.4% .It is a novel application of AuNPs-based probe for PFOS detection .The proposed method has more advanta-ges such as rapidity ,low-cost and simplicity than conventional ones .In addition ,it has the visual sensing function and the differ-ence of color can be sensed by naked eyes directly ,which produce ideas of real-time colorimetric strategies of nanoprobe applica-tion in environmental pollutant detection .
