CAJ | 학술논문

通过在好氧颗粒污泥培养过程中分别在第1～7天和第8～14天投加聚合氯化铝（PAC）的方式，分析颗粒的形成过程，并研究胞外聚合物总量及其各组分的含量变化和空间分布。研究发现：第8～14天投加PAC的反应器形成颗粒时间提前了6天，且形成的颗粒外形规则，大小均匀，去除污染物性能良好；反应器中胞外多聚物和蛋白质含量总体较多，且随颗粒的形成而增加，之后略有下降并在颗粒成熟后维持稳定，蛋白质/多糖的值在颗粒形成期均增加超过2.5倍，有利于颗粒污泥的形成；多重荧光染色表明：两个反应器中PAC投加时间不同，β-D-呋喃葡萄糖、脂类和活细胞均呈现不同的分布情况，但对蛋白质和α-呋喃葡萄糖、α-甘露糖分布的影响较小。
통과재호양과립오니배양과정중분별재제1～7천화제8～14천투가취합록화려（PAC）적방식，분석과립적형성과정，병연구포외취합물총량급기각조분적함량변화화공간분포。연구발현：제8～14천투가PAC적반응기형성과립시간제전료6천，차형성적과립외형규칙，대소균균，거제오염물성능량호；반응기중포외다취물화단백질함량총체교다，차수과립적형성이증가，지후략유하강병재과립성숙후유지은정，단백질/다당적치재과립형성기균증가초과2.5배，유리우과립오니적형성；다중형광염색표명：량개반응기중PAC투가시간불동，β-D-부남포도당、지류화활세포균정현불동적분포정황，단대단백질화α-부남포도당、α-감로당분포적영향교소。
This paper analyzed the formation process of granules,and researched the content changes and the spatial distribution of extracellular polymeric substances by adding poly aluminium chloride in 1—7 days and 8—14 days respectively during the process of cultivating aerobic granular sludge. The granules formation time of the reactor adding PAC in 8—14 days was 6 days ahead of time,and the sludge granules had regular shape,uniform size,and good contaminants removal performance. Moreover,the reactor extracellular polymeric substances and proteins content was higher,and increased with the formation of granules and then decreased slightly,and stayed stable after the granules matured. The value of proteins/polysaccharides during the process of granules formation increased by more than 2.5 times,which was conducive to the formation of granular sludge. Multiple fluorescent staining showed that different PAC dosing times between the reactors led to different distributions ofβ-polysaccharides and lipids and live cells,but had smaller effect on the distribution of proteins andα-polysaccharides .