CAJ | 학술논문

目的探讨血小板（PL T ）荧光色素染色方法PL T‐F通道在低值血小板测定中的临床应用意义。方法利用XN‐1000全自动血细胞分析仪PL T‐F通道检测103例患者低值血小板标本（≤50×109／L ），同时用N E‐2100血细胞分析仪、手工显微镜计数法进行检测。数据比较用直线相关回归分析。结果低值血小板检测重复性 CV 值在 XE2100 PLT‐I通道为6．7％～16．5％，PLT‐O 通道为7．1％～15．6％，XN‐1000的PLT‐F通道为4．9％～9．5％；以显微镜手工计数为参考方法，PLT‐I、PLT‐O、PLT‐F通道相关系数（r）分别为0．900、0．929、0．959。结论 PL T‐F通道在低值血小板测定中准确性较好，应用价值较大。
목적 탐토혈소판（PL T ）형광색소염색방법PL T‐F통도재저치혈소판측정중적림상응용의의。방법 이용XN‐1000전자동혈세포분석의PL T‐F통도검측103례환자저치혈소판표본（≤50×109／L ），동시용N E‐2100혈세포분석의、수공현미경계수법진행검측。수거비교용직선상관회귀분석。결과 저치혈소판검측중복성 CV 치재 XE2100 PLT‐I통도위6．7％～16．5％，PLT‐O 통도위7．1％～15．6％，XN‐1000적PLT‐F통도위4．9％～9．5％；이현미경수공계수위삼고방법，PLT‐I、PLT‐O、PLT‐F통도상관계수（r）분별위0．900、0．929、0．959。결론 PL T‐F통도재저치혈소판측정중준학성교호，응용개치교대。
Objective] To explore the significance of PLT‐fluorescence(PLT‐F) channel in the determination of low‐count platelet .[Methods]Totally 103 patients with low‐count platelet(≤50 × 109/L) were analyzed by PLT‐F channel with XN‐1000 automated hematology analyzer .Meanwhile ,NE‐2100 hematology analyzer and hand‐made microscopic count method were used for the detection .The data were analyzed with straight line correlation regression analysis .[Results] The CV value of reproducibility of low‐count platelet in XE2100 PLT‐I ,PLT‐O and PLT‐F channels were 6 .7~16 .5% ,7 .1~15 .6% and 4 .9~9 .5% ,respectively .The coefficient correlation(r) of PLT‐I , PLT‐O and PLT‐F by hand‐made microscopic count reference method were 0 .900 ,0 .929 and 0 .959 ,respectively .[Conclusion] PLT‐F channel in the determination of low‐count platelet has good accuracy and high application value .