医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2014年
11期
2081-2083
,共3页
DNA/免疫学%红斑狼疮,系统性/诊断%抗体,抗核/分析
DNA/免疫學%紅斑狼瘡,繫統性/診斷%抗體,抗覈/分析
DNA/면역학%홍반랑창,계통성/진단%항체,항핵/분석
DNA/IM%Lupus Erythematosus,Systemic/DI%Antibodies,Antinuclear/AN
目的 探讨抗核抗体（ANA）、抗核抗体谱（ANAs）及抗双链DNA抗体（抗dsDNA抗体）的联合检测对系统性红斑狼疮（SLE）的诊断价值。方法 选择2012年1月至2014年1月间泉州市某市级医院收治的SLE患者150例作为SLE组，同期收治的其他风湿性疾病患者30例作为疾病对照组，门诊体检健康者30例作为正常对照组。应用间接免疫荧光法检测血清 ANA ，应用免疫印迹法检测血清ANAs和抗dsDNA抗体水平。结果 SLE组ANA、抗dsDNA抗体、抗AHA、抗nRNP、抗AnuA、抗Sm阳性率显著高于疾病对照组和健康对照组（ P ＜0．01）。S L E患者中仅5．33％的患者出现单项抗体阳性，其余94．67％的患者一次性检测出2项或以上抗体阳性。SLE患者治疗后ANA、抗dsDNA抗体、抗AHA、抗Sm和抗SS‐A阳性率均不同程度下降，其中ANA、抗dsDNA抗体、抗AHA好转率分别为23．33％、83．33％和63．33％。结论 SLE患者ANA、ANAs和抗ds‐DNA抗体阳性率较高，联合检测和动态观察 ANA、ANAs和抗 ds‐DNA抗体对SLE诊断和治疗具有一定意义。
目的 探討抗覈抗體（ANA）、抗覈抗體譜（ANAs）及抗雙鏈DNA抗體（抗dsDNA抗體）的聯閤檢測對繫統性紅斑狼瘡（SLE）的診斷價值。方法 選擇2012年1月至2014年1月間泉州市某市級醫院收治的SLE患者150例作為SLE組，同期收治的其他風濕性疾病患者30例作為疾病對照組，門診體檢健康者30例作為正常對照組。應用間接免疫熒光法檢測血清 ANA ，應用免疫印跡法檢測血清ANAs和抗dsDNA抗體水平。結果 SLE組ANA、抗dsDNA抗體、抗AHA、抗nRNP、抗AnuA、抗Sm暘性率顯著高于疾病對照組和健康對照組（ P ＜0．01）。S L E患者中僅5．33％的患者齣現單項抗體暘性，其餘94．67％的患者一次性檢測齣2項或以上抗體暘性。SLE患者治療後ANA、抗dsDNA抗體、抗AHA、抗Sm和抗SS‐A暘性率均不同程度下降，其中ANA、抗dsDNA抗體、抗AHA好轉率分彆為23．33％、83．33％和63．33％。結論 SLE患者ANA、ANAs和抗ds‐DNA抗體暘性率較高，聯閤檢測和動態觀察 ANA、ANAs和抗 ds‐DNA抗體對SLE診斷和治療具有一定意義。
목적 탐토항핵항체（ANA）、항핵항체보（ANAs）급항쌍련DNA항체（항dsDNA항체）적연합검측대계통성홍반랑창（SLE）적진단개치。방법 선택2012년1월지2014년1월간천주시모시급의원수치적SLE환자150례작위SLE조，동기수치적기타풍습성질병환자30례작위질병대조조，문진체검건강자30례작위정상대조조。응용간접면역형광법검측혈청 ANA ，응용면역인적법검측혈청ANAs화항dsDNA항체수평。결과 SLE조ANA、항dsDNA항체、항AHA、항nRNP、항AnuA、항Sm양성솔현저고우질병대조조화건강대조조（ P ＜0．01）。S L E환자중부5．33％적환자출현단항항체양성，기여94．67％적환자일차성검측출2항혹이상항체양성。SLE환자치료후ANA、항dsDNA항체、항AHA、항Sm화항SS‐A양성솔균불동정도하강，기중ANA、항dsDNA항체、항AHA호전솔분별위23．33％、83．33％화63．33％。결론 SLE환자ANA、ANAs화항ds‐DNA항체양성솔교고，연합검측화동태관찰 ANA、ANAs화항 ds‐DNA항체대SLE진단화치료구유일정의의。
Objective] To explore the value of antinuclear antibody(ANA) ,antinuclear antibody profile(ANAs) and anti‐double stranded DNA antibody (anti dsDNA antibody ) in the diagnosis of systemic lupus erythematosus (SLE) .[Methods] Totally 150 patients with SLE in a municipal hospital of Quanzhou city from Jan .2012 to Jan . 2014 were selected as SLE group ,and other 30 patients with rheumatic diseases were selected as disease control group ,and 30 health examination persons were selected as normal control group .Serum ANA was detected by indi‐rect immunofluorescence method .Serum ANAs and anti dsDNA antibody were measured by western blotting .[Re‐sults] The positive rates of serum ANA ,anti dsDNA antibody ,anti‐AHA ,anti‐nRNP ,anti‐AnuA and anti‐Sm in SLE group were significantly higher than those in disease control group and healthy control group( P <0 .01) .In SLE group ,only 5 .33% of patients had single positive antibody ,and the remaining 94 .67% of patients had two or more positive antibodies .After treatment ,the positive rates of ANA ,anti dsDNA antibody ,anti‐AHA ,anti‐Sm and anti‐SS‐A in SLE patients were decreased in different degree .The improvement rate of ANA ,anti‐dsDNA antibody and anti‐AHA were 23 .33% ,83 .33% and 63 .33% ,respectively .[Conclusion] The positive rates of ANA ,ANAs and anti ds‐DNA antibody in SLE patients is higher .The joint detection and dynamic observation of ANA ,ANAs and anti‐ds‐DNA antibody is of significance in the diagnosis and treatment of SLE .
