河南科学
河南科學
하남과학
HENAN SCIENCE
2014年
11期
2244-2247
,共4页
张秀江%兰芳菲%胡虹%王传兴
張秀江%蘭芳菲%鬍虹%王傳興
장수강%란방비%호홍%왕전흥
里氏木霉%菌株选育%紫外线%亚硝酸
裏氏木黴%菌株選育%紫外線%亞硝痠
리씨목매%균주선육%자외선%아초산
Trichoderma reesei%strains breeding%ultraviolet%nitrous alid
采用紫外线和亚硝酸联合诱变技术,得到产纤维素酶、木聚糖酶、β-葡聚糖酶的高产的菌株里氏木霉Y07.试验表明,诱变后菌株的木聚糖酶活由325 U/g,提高到28500 U/g,相对于出发菌株提高了88倍;纤维素酶活由560 U/g,提高到2600 U/g,相对于出发菌株提高了4.6倍;β-葡聚糖酶活由480 U/g,提高到5500 U/g,相对于出发菌株提高了11.5倍.
採用紫外線和亞硝痠聯閤誘變技術,得到產纖維素酶、木聚糖酶、β-葡聚糖酶的高產的菌株裏氏木黴Y07.試驗錶明,誘變後菌株的木聚糖酶活由325 U/g,提高到28500 U/g,相對于齣髮菌株提高瞭88倍;纖維素酶活由560 U/g,提高到2600 U/g,相對于齣髮菌株提高瞭4.6倍;β-葡聚糖酶活由480 U/g,提高到5500 U/g,相對于齣髮菌株提高瞭11.5倍.
채용자외선화아초산연합유변기술,득도산섬유소매、목취당매、β-포취당매적고산적균주리씨목매Y07.시험표명,유변후균주적목취당매활유325 U/g,제고도28500 U/g,상대우출발균주제고료88배;섬유소매활유560 U/g,제고도2600 U/g,상대우출발균주제고료4.6배;β-포취당매활유480 U/g,제고도5500 U/g,상대우출발균주제고료11.5배.
The mutant strain Y07 capable of producing high-yield cellulase,xylanase,β-glucanse was obtained through ultraviolet light and nitrosoguanidine mutation of Trichoderma reesei. The xylanase activity of strain Y07 was 28500 U/g,which increased 88 times compared with wild-type strain of 325 U/g. The cellulase activity of strain Y07 was 2600 U/g,which increased 4.6 times compared with wild-type strain of 560 U/g. Theβ-glucanas activity of strain Y07 was 5500 U/g,which increased 11.5 times compared with wild-type strain of 480 U/g.