河南科学
河南科學
하남과학
HENAN SCIENCE
2014年
11期
2237-2239
,共3页
王玉金%杨书豪%刘丽%李珊珊
王玉金%楊書豪%劉麗%李珊珊
왕옥금%양서호%류려%리산산
亚硝酸钠氧化法%总胆红素%试剂研制
亞硝痠鈉氧化法%總膽紅素%試劑研製
아초산납양화법%총담홍소%시제연제
sodium nitrite oxidation method%total bilirubin%reagent development
探讨建立一种亚硝酸钠氧化法测定血清总胆红素的新方法.采用亚硝酸钠氧化法测定血清总胆红素反应前后吸光度的变化，与标准品相比较，求得标本中总胆红素的浓度.精密度试验批内精密度（CV）值为1.38％；批间CV值为1.74％.线性范围可达792μmol/L .与其他相关试剂比较γ=0.9967.可用于检测临床标本.
探討建立一種亞硝痠鈉氧化法測定血清總膽紅素的新方法.採用亞硝痠鈉氧化法測定血清總膽紅素反應前後吸光度的變化，與標準品相比較，求得標本中總膽紅素的濃度.精密度試驗批內精密度（CV）值為1.38％；批間CV值為1.74％.線性範圍可達792μmol/L .與其他相關試劑比較γ=0.9967.可用于檢測臨床標本.
탐토건립일충아초산납양화법측정혈청총담홍소적신방법.채용아초산납양화법측정혈청총담홍소반응전후흡광도적변화，여표준품상비교，구득표본중총담홍소적농도.정밀도시험비내정밀도（CV）치위1.38％；비간CV치위1.74％.선성범위가체792μmol/L .여기타상관시제비교γ=0.9967.가용우검측림상표본.
A new sodium nitrite oxidation method for measuring serum total bilirubin was establised. Sodium nitrite oxidation method was applied for detecting serum total bilirubin(TBIL)concentrations according to the absorbance changes,and the results were analysed,compared with the standard controls. The intra-assay CV were 1.38%. The interassay CV were 1.74%. The linear ranges were up to 792μmol/L. The sodium nitrite method was well correlated with Vanadate method and Diazo method. It can be used in routine detections of serum total TBIL.
