CAJ | 학술논문

目的：建立高效液相色谱法测定人血浆中拉莫三嗪的浓度。方法以DiamonsilTM C18反相柱（150mm ×4.6mm，5μm）为色谱柱，流动相为0.03mol· L －1醋酸铵-甲醇（58∶42）；流速：1.0mL· min －1；柱温：30℃；检测波长：310nm。以乙酸乙酯为提取剂。结果拉莫三嗪浓度在0.5～24.0μg· mL －1范围内，峰面积与浓度线性关系良好；拉莫三嗪的低、中、高浓度（1.0，8.0，20.0μg· mL －1）相对平均回收率大于95％；提取回收率大于75％。日内、日间RSD均低于10％（n＝5）。分析方法的检测限0.1μg· mL －1；拉莫三嗪曲线回归方程为：Y＝0.0899X＋0.0215，r＝0.9989（n＝7）；结论该方法灵敏、准确、简单、快速，能够满足体内药物分析的要求。
목적：건립고효액상색보법측정인혈장중랍막삼진적농도。방법이DiamonsilTM C18반상주（150mm ×4.6mm，5μm）위색보주，류동상위0.03mol· L －1작산안-갑순（58∶42）；류속：1.0mL· min －1；주온：30℃；검측파장：310nm。이을산을지위제취제。결과랍막삼진농도재0.5～24.0μg· mL －1범위내，봉면적여농도선성관계량호；랍막삼진적저、중、고농도（1.0，8.0，20.0μg· mL －1）상대평균회수솔대우95％；제취회수솔대우75％。일내、일간RSD균저우10％（n＝5）。분석방법적검측한0.1μg· mL －1；랍막삼진곡선회귀방정위：Y＝0.0899X＋0.0215，r＝0.9989（n＝7）；결론해방법령민、준학、간단、쾌속，능구만족체내약물분석적요구。
OBJECTIVE To establish a method for determining the concentration of lamotrigine in human plas-ma by HPLC.METHODS The drug from plasma was analyzed in a reverse phase HPLC system C18 column (150mm ×4.6mm,5μm);mobile phase consisted of 0.03mol· L-1 ammonium acetate-methanol(58∶42);the flow rate was1.0mL · min-1;the detection wavelength was at 310nm.Ethyl acetate was used as extracting sol-vent.RESULTS The peak area and concentration of Lamotrigine in 0.5~24.0μg· mL-1 has good linearity.The average recoveries of lamotrigne in low,middle and high concentrations (1.0,8.0,20.0μg· mL-1 ) were over 95%respectively;the extraction recovery over 75%.The intra-day and inter-day variation ( RSD) was less than 10%( n=5).The minimum detectable concentration of lamotrigne was 0.1μg · mL-1.The regression equation was Y =0.0899X+0.0215.r=0.9989(n=7).CONCLUSION The method is sensitive,accurate,simple,fast.It is suit-able for deter mination of drug analysis.