黑龙江医学
黑龍江醫學
흑룡강의학
HEILONGJIANG MEDICAL JOURNAL
2014年
11期
1250-1251,1252
,共3页
胶乳增强免疫比浊法%心型脂肪酸结合蛋白%急性心肌梗死%方法学评价
膠乳增彊免疫比濁法%心型脂肪痠結閤蛋白%急性心肌梗死%方法學評價
효유증강면역비탁법%심형지방산결합단백%급성심기경사%방법학평개
Latex-enhanced turbidimetric immunoassay%Heart-type fatty acid binding protein%AMI%Methodological evaluation
目的：对胶乳增强免疫比浊法定量测定血清心型脂肪酸结合蛋白（ H－FABP）的方法进行临床实验评价。方法分析胶乳增强免疫比浊法测定H－FABP的精密度、线性范围、前带反应、回收率和干扰因素，并考察其与酶联免疫吸附法的相关性。结果胶乳增强免疫比浊法测定血清H－FABP批内精密度的高值与低值变异系数分别为0．54％、1．56％；批间精密度的高值与低值变异系数分别为0．58％、1．8％，回收率为96．2％～99．3％。线性测定良好，可检测范围为2．5 ng／mL～160 ng／mL，标准品曲线回归方程为Y＝1．02X＋0．31，标准品检测相关性R2≥0．995。跟国外同类试剂相比较，曲线回归方程为Y＝0．97X＋0．13，检测相关性R2＝0．998。实验组血清H－FABP检测结果为（78．3±10．4）ng／mL，与对照组相比差异有统计学意义。结论胶乳增强免疫比浊法测定血清H－FABP快速、准确、可靠，适用于临床急性心肌梗死的早期诊断。
目的：對膠乳增彊免疫比濁法定量測定血清心型脂肪痠結閤蛋白（ H－FABP）的方法進行臨床實驗評價。方法分析膠乳增彊免疫比濁法測定H－FABP的精密度、線性範圍、前帶反應、迴收率和榦擾因素，併攷察其與酶聯免疫吸附法的相關性。結果膠乳增彊免疫比濁法測定血清H－FABP批內精密度的高值與低值變異繫數分彆為0．54％、1．56％；批間精密度的高值與低值變異繫數分彆為0．58％、1．8％，迴收率為96．2％～99．3％。線性測定良好，可檢測範圍為2．5 ng／mL～160 ng／mL，標準品麯線迴歸方程為Y＝1．02X＋0．31，標準品檢測相關性R2≥0．995。跟國外同類試劑相比較，麯線迴歸方程為Y＝0．97X＋0．13，檢測相關性R2＝0．998。實驗組血清H－FABP檢測結果為（78．3±10．4）ng／mL，與對照組相比差異有統計學意義。結論膠乳增彊免疫比濁法測定血清H－FABP快速、準確、可靠，適用于臨床急性心肌梗死的早期診斷。
목적：대효유증강면역비탁법정량측정혈청심형지방산결합단백（ H－FABP）적방법진행림상실험평개。방법분석효유증강면역비탁법측정H－FABP적정밀도、선성범위、전대반응、회수솔화간우인소，병고찰기여매련면역흡부법적상관성。결과효유증강면역비탁법측정혈청H－FABP비내정밀도적고치여저치변이계수분별위0．54％、1．56％；비간정밀도적고치여저치변이계수분별위0．58％、1．8％，회수솔위96．2％～99．3％。선성측정량호，가검측범위위2．5 ng／mL～160 ng／mL，표준품곡선회귀방정위Y＝1．02X＋0．31，표준품검측상관성R2≥0．995。근국외동류시제상비교，곡선회귀방정위Y＝0．97X＋0．13，검측상관성R2＝0．998。실험조혈청H－FABP검측결과위（78．3±10．4）ng／mL，여대조조상비차이유통계학의의。결론효유증강면역비탁법측정혈청H－FABP쾌속、준학、가고，괄용우림상급성심기경사적조기진단。
Objective To do clinical experimental evaluation on serum heart-type fatty acid binding protein ( H-FABP) immunoas-say method for latex-enhanced turbidimetric immunoassay.Methods H-FABP immunoassay precision latex-enhanced turbidimetry, linear range, response, recoveriy rate and confounding factors were analyzed, and the relationship between ELISA and it was examed.Re-sults The high and low values CV%of the latex-enhanced immunoturbidimetric assay serum H-FABP assay precision were 0.54%and 1.56%;inter-assay precision CV% of high and low values were 0.58% and 1.8%, and the recovery was 96.2%~99.3%.Linear measurement was good, and could be detected in the range of 2.5 ng/mL~160 ng/mL.The standard curve regression equation was Y=1. 02X+0.31, and standard detection correlation was R2≥0.995.Compared with similar foreign agents, curve regression equation was Y=0.97X+0.13, with a correlation R2 =0.998.The serum H-FABP test result was (78.3 ±10.4) ng/mL, with statistically significant differences with the control group.Conclusion Immunoturbidimetric assay of serum H-FABP latex-enhancement is fast, accurate, relia-ble and suitable for clinical evaluation of acute myocardial infarction.
