黑龙江医学
黑龍江醫學
흑룡강의학
HEILONGJIANG MEDICAL JOURNAL
2014年
11期
1242-1244
,共3页
吉西他滨%乳腺肿瘤%MCF-7细胞%MTA1 mRNA%基因%肿瘤转移
吉西他濱%乳腺腫瘤%MCF-7細胞%MTA1 mRNA%基因%腫瘤轉移
길서타빈%유선종류%MCF-7세포%MTA1 mRNA%기인%종류전이
Gemcitabine%Breast neoplasms%MCF-7 cell%MTA1m RNA%Gene%Tumor metastasis
目的：探讨吉西他滨对体外培养的乳腺肿瘤MCF－7细胞株抑制作用及其作用机制。方法将浓度为25 mg／mL、50 mg／mL、100 mg／mL、200 mg／mL和400 mg／mL吉西他滨培养液分为5组：A组、B组、C组、D组和E组。 A组、B组、C组、D组和E组均作用于体外传代培养对数生长期乳腺肿瘤细胞株MCF－7细胞24 h；使用四甲基偶氮唑盐（ MTT）法检测5组各对乳腺肿瘤细胞株MCF－7的生长抑制率；用实时定量聚合酶链反应（ PCR）检测5组各对乳腺肿瘤MCF－7细胞株肿瘤转移相关基因1（MTA1）mRNA的相对表达量。结果 A组、B组、C组、D组和E组对乳腺肿瘤MCF－7细胞株抑制率A组＜B组＜C组＜D组＜E组，差异均有统计学意义（P＜0．05）；C组、D组和E组MTA1 mRNA的相对表达量均人低于空白对照组，且这3组的MTA1 mR－NA的相对表达量依次降低，差异均有统计学意义（P＜0．01）。结论吉西他滨能抑制乳腺肿瘤MCF－7细胞株增殖，且有剂量依赖性，其主要作用机制是通过下调MTA1 mRNA的表达，从而发挥抑制乳腺肿瘤MCF－7细胞株转移与抗乳腺肿瘤作用。
目的：探討吉西他濱對體外培養的乳腺腫瘤MCF－7細胞株抑製作用及其作用機製。方法將濃度為25 mg／mL、50 mg／mL、100 mg／mL、200 mg／mL和400 mg／mL吉西他濱培養液分為5組：A組、B組、C組、D組和E組。 A組、B組、C組、D組和E組均作用于體外傳代培養對數生長期乳腺腫瘤細胞株MCF－7細胞24 h；使用四甲基偶氮唑鹽（ MTT）法檢測5組各對乳腺腫瘤細胞株MCF－7的生長抑製率；用實時定量聚閤酶鏈反應（ PCR）檢測5組各對乳腺腫瘤MCF－7細胞株腫瘤轉移相關基因1（MTA1）mRNA的相對錶達量。結果 A組、B組、C組、D組和E組對乳腺腫瘤MCF－7細胞株抑製率A組＜B組＜C組＜D組＜E組，差異均有統計學意義（P＜0．05）；C組、D組和E組MTA1 mRNA的相對錶達量均人低于空白對照組，且這3組的MTA1 mR－NA的相對錶達量依次降低，差異均有統計學意義（P＜0．01）。結論吉西他濱能抑製乳腺腫瘤MCF－7細胞株增殖，且有劑量依賴性，其主要作用機製是通過下調MTA1 mRNA的錶達，從而髮揮抑製乳腺腫瘤MCF－7細胞株轉移與抗乳腺腫瘤作用。
목적：탐토길서타빈대체외배양적유선종류MCF－7세포주억제작용급기작용궤제。방법장농도위25 mg／mL、50 mg／mL、100 mg／mL、200 mg／mL화400 mg／mL길서타빈배양액분위5조：A조、B조、C조、D조화E조。 A조、B조、C조、D조화E조균작용우체외전대배양대수생장기유선종류세포주MCF－7세포24 h；사용사갑기우담서염（ MTT）법검측5조각대유선종류세포주MCF－7적생장억제솔；용실시정량취합매련반응（ PCR）검측5조각대유선종류MCF－7세포주종류전이상관기인1（MTA1）mRNA적상대표체량。결과 A조、B조、C조、D조화E조대유선종류MCF－7세포주억제솔A조＜B조＜C조＜D조＜E조，차이균유통계학의의（P＜0．05）；C조、D조화E조MTA1 mRNA적상대표체량균인저우공백대조조，차저3조적MTA1 mR－NA적상대표체량의차강저，차이균유통계학의의（P＜0．01）。결론길서타빈능억제유선종류MCF－7세포주증식，차유제량의뢰성，기주요작용궤제시통과하조MTA1 mRNA적표체，종이발휘억제유선종류MCF－7세포주전이여항유선종류작용。
Objective To investigate the inhibitory effect and mechanism of action of gemcitabine on breast cancer cell line MCF-7 in vitro.Methods The concentrations of 25, 50, 100, 200 and 400mg/ml gemcitabine culture were divided into five groups: A, B, C, D and E groups.A, B, C, D and E groups were in vitro culture of logarithmic growth phase of breast tumor cell line MCF-7 for 24h;methyl thiazolyl tetrazolium ( MTT) assay was used for the detection of 5 groups of breast tumor cell line MCF-7 growth inhibition rate;real-time quantitative polymerase chain reaction ( PCR) was used to detect the relative expression of mRNA of breast cancer MCF-7 cell line metas-tasis associated gene 1 (MTA1) in 5 groups.Results In A, B, C, D and E in breast cancer cell line MCF-7 inhibition rate of A group<B group <C group <D group <E group, the differences were statistically significant (P<0.05);the relative expressions of C group, D group and E group MTA1 mRNA were lower than the blank control group.MTA1 mRNA relative expressions quantity of the three groups reduced, and the differences were statistically significant ( P <0.01).Conclusion Gemcitabine can inhibit the proliferation of breast cancer cell line MCF-7, and has dose dependent.Its main mechanism inhibits breast cancer cell line MCF-7 and the role of anti breast cancer metastasis through the down-regulation of MTA1 mRNA expression.
