CAJ | 학술논문

目的：制备复方黄芩温敏凝胶，并建立了温敏凝胶中黄芩苷含量测定的质量标准。方法以泊洛沙姆作为凝胶材料，考察含有不同泊洛沙姆407（Pluronic F127）和泊洛沙姆188（Pluronic F68）浓度配比的处方对凝胶胶凝温度的影响。采用高效液相色谱法测定凝胶中黄芩苷的含量：色谱柱为Acclaim 120 C18柱（4.6mm ×250mm，5μm）；流动相为甲醇-水-磷酸（57∶43∶0.2），流速1.0mL· min －1；检测波长280nm，柱温30℃。结果选择胶凝温度为29.5℃的凝胶基质：22％P407／8％P188。黄芩苷在0.0253～0.1508（mg· mL －1）范围内线性关系良好，平均加样回收率为103.74％，RSD为1.26％。结论泊洛沙姆温敏凝胶的制备工艺简便易行，筛选得到的凝胶处方适于临床皮肤给药。所建立的质量标准结果准确，可靠，重现性好，可用于复方黄芩温敏凝胶的质量控制。
목적：제비복방황금온민응효，병건립료온민응효중황금감함량측정적질량표준。방법이박락사모작위응효재료，고찰함유불동박락사모407（Pluronic F127）화박락사모188（Pluronic F68）농도배비적처방대응효효응온도적영향。채용고효액상색보법측정응효중황금감적함량：색보주위Acclaim 120 C18주（4.6mm ×250mm，5μm）；류동상위갑순-수-린산（57∶43∶0.2），류속1.0mL· min －1；검측파장280nm，주온30℃。결과선택효응온도위29.5℃적응효기질：22％P407／8％P188。황금감재0.0253～0.1508（mg· mL －1）범위내선성관계량호，평균가양회수솔위103.74％，RSD위1.26％。결론박락사모온민응효적제비공예간편역행，사선득도적응효처방괄우림상피부급약。소건립적질량표준결과준학，가고，중현성호，가용우복방황금온민응효적질량공제。
OBJECTIVE To prepare the preparation of compound Huangqin temperature sensitive gel,and to establish the quality control standard to determine the content of baicalin temperature sensitive gel.METHODS Bo Losham was choosed as gel material,the influence of different concentration ratio of Bo Losham 407(Pluronic F127) and Bo Losham 188(Pluronic F68) on the prescription on the gel temperature was investigatal.HPLC method was es-tablished for determination of Baicalin in gel:the chromatographic column was Acclaim 120 C18 column(4.6mm × 250mm,5μm);the mobile phase of methanol water phosphoric acid(57∶43∶0.2),the flow rate was 1.0mL· min -1;the detection wavelength was 280 nm and the column temperature was 30℃.RESULTS The gelation temperature was 29.5℃gel matrix:22% P407/8% P188.Baicalin in 0.0253~0.1508 ( mg · mL-1 ) the linear relationship is good,the average recovery was 103.74%,RSD was 1.26%.CONCLUSION The poloxamer temperature sensitive gel preparation process is simple,the prescription screening gelobtained is suitable for clinical skin medication.The quality standard is accurate,reliable,reproducible and can be used for quality control of compound Huangqin temper-ature sensitive gel.