海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
11期
56-57
,共2页
HPLC%蚕豆花%槲皮素%山奈酚%含量测定
HPLC%蠶豆花%槲皮素%山奈酚%含量測定
HPLC%잠두화%곡피소%산내분%함량측정
HPLC%Broadbean flower%Quercetin%Kamepferol%Content determination
目的：建立HPLC法测定蚕豆花中的槲皮素和山奈酚含量的方法。方法测定槲皮素和山奈酚的色谱条件为色谱柱 InertSustain C18（250mm ×4.6mm，5μm）；以乙腈（A）∶0.4％磷酸水溶液（B）为流动相进行梯度洗脱，流速为0.8mL· min －1；检测波长为365nm；柱温为30℃。结果槲皮素进样量在0.034～0.272μg范围内（r＝0.9999），山奈酚进样量在0.040～0.317μg范围内（r＝0.9998）线性关系良好，平均回收率槲皮素为99.20％（RSD％＝0.81，n＝6），山奈酚为98.39％（RSD％＝1.49，n＝6）。结论本法操作简便快捷、稳定性高、重现性好，为蚕豆花药材的质量评价提供依据。
目的：建立HPLC法測定蠶豆花中的槲皮素和山奈酚含量的方法。方法測定槲皮素和山奈酚的色譜條件為色譜柱 InertSustain C18（250mm ×4.6mm，5μm）；以乙腈（A）∶0.4％燐痠水溶液（B）為流動相進行梯度洗脫，流速為0.8mL· min －1；檢測波長為365nm；柱溫為30℃。結果槲皮素進樣量在0.034～0.272μg範圍內（r＝0.9999），山奈酚進樣量在0.040～0.317μg範圍內（r＝0.9998）線性關繫良好，平均迴收率槲皮素為99.20％（RSD％＝0.81，n＝6），山奈酚為98.39％（RSD％＝1.49，n＝6）。結論本法操作簡便快捷、穩定性高、重現性好，為蠶豆花藥材的質量評價提供依據。
목적：건립HPLC법측정잠두화중적곡피소화산내분함량적방법。방법측정곡피소화산내분적색보조건위색보주 InertSustain C18（250mm ×4.6mm，5μm）；이을정（A）∶0.4％린산수용액（B）위류동상진행제도세탈，류속위0.8mL· min －1；검측파장위365nm；주온위30℃。결과곡피소진양량재0.034～0.272μg범위내（r＝0.9999），산내분진양량재0.040～0.317μg범위내（r＝0.9998）선성관계량호，평균회수솔곡피소위99.20％（RSD％＝0.81，n＝6），산내분위98.39％（RSD％＝1.49，n＝6）。결론본법조작간편쾌첩、은정성고、중현성호，위잠두화약재적질량평개제공의거。
OBJECTIVE To establish a analytical method on content determination of queretin and kaempferol in broadbean flower by HPLC.METHODS The chromatographic conditions were:column:InertSustain C18 (250mm ×4.6mm,5μm);mobile phase:acetonitrile (A)-0.4% phosphoric acid solution (B) (gradient elution),flow rate at 0.8mL· min -1,and detection wavelength at 365nm,column temperature was 30℃.RESULTS The injection volume of quercetin was at the range of 0.034~0.272μg( r=0.9999).The injection volume of kamepferol showed a good linear relationship at the range of 0.040~0.317μg( r=0.9998) ,The average recovery was 99.20%quercetin for (RSD%=0.81,n=6) and 98.39% for kamepferol (RSD%=1.49,n=6).CONCLUSION The method is simple and fast,which has high stability and good reproducibility and provide a basis for quality evaluating for broad-bean flower as medicinal materials.
