医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
1期
48-49
,共2页
大黄素%miR-146a%肺泡巨噬细胞%炎症反应
大黃素%miR-146a%肺泡巨噬細胞%炎癥反應
대황소%miR-146a%폐포거서세포%염증반응
Emodin%miR-146a%Alveolar macrophage%Inflammatory response
目的：探讨微小RNA-146a(miR-146a)在大黄素对脂多糖(LPS)诱导的肺泡巨噬细胞炎症反应中的作用。方法将体外去致热源培养的大鼠肺泡巨噬细胞株NR8383分为空白对照组、LPS处理组和大黄素+LPS处理组,细胞培养6 h后收集细胞,采用实时荧光定量逆转录-聚合酶链反应(RT-qPCR)检测细胞中miR-146a的表达,Western blot法检测细胞中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的表达。结果与空白对照组相比较,LPS作用后细胞中miR-146a、TNF-α和IL-6表达量明显升高；与LPS处理组相比较,大黄素+LPS处理组中miR-146a表达量显著上调,而TNF-α和IL-6表达量显著下调。结论大黄素可上调肺泡巨噬细胞中miR-146a的表达,大黄素可抑制巨噬细胞中TNF-α和IL-6的表达,miR-146a可能参与调控大黄素抗肺泡巨噬细胞炎症反应过程。
目的：探討微小RNA-146a(miR-146a)在大黃素對脂多糖(LPS)誘導的肺泡巨噬細胞炎癥反應中的作用。方法將體外去緻熱源培養的大鼠肺泡巨噬細胞株NR8383分為空白對照組、LPS處理組和大黃素+LPS處理組,細胞培養6 h後收集細胞,採用實時熒光定量逆轉錄-聚閤酶鏈反應(RT-qPCR)檢測細胞中miR-146a的錶達,Western blot法檢測細胞中腫瘤壞死因子-α(TNF-α)和白細胞介素-6(IL-6)的錶達。結果與空白對照組相比較,LPS作用後細胞中miR-146a、TNF-α和IL-6錶達量明顯升高；與LPS處理組相比較,大黃素+LPS處理組中miR-146a錶達量顯著上調,而TNF-α和IL-6錶達量顯著下調。結論大黃素可上調肺泡巨噬細胞中miR-146a的錶達,大黃素可抑製巨噬細胞中TNF-α和IL-6的錶達,miR-146a可能參與調控大黃素抗肺泡巨噬細胞炎癥反應過程。
목적：탐토미소RNA-146a(miR-146a)재대황소대지다당(LPS)유도적폐포거서세포염증반응중적작용。방법장체외거치열원배양적대서폐포거서세포주NR8383분위공백대조조、LPS처리조화대황소+LPS처리조,세포배양6 h후수집세포,채용실시형광정량역전록-취합매련반응(RT-qPCR)검측세포중miR-146a적표체,Western blot법검측세포중종류배사인자-α(TNF-α)화백세포개소-6(IL-6)적표체。결과여공백대조조상비교,LPS작용후세포중miR-146a、TNF-α화IL-6표체량명현승고；여LPS처리조상비교,대황소+LPS처리조중miR-146a표체량현저상조,이TNF-α화IL-6표체량현저하조。결론대황소가상조폐포거서세포중miR-146a적표체,대황소가억제거서세포중TNF-α화IL-6적표체,miR-146a가능삼여조공대황소항폐포거서세포염증반응과정。
Objective To investigate the role of microR-146a (miR-146a) in the lipopolysaccharide (LPS)-induced inflammation of rat alveolar macrophages by emodin. Methods The rat alveolar macrophages NR8383 cultured without pyrogen in vitro were divided into three groups: blank control group; LPS-stimulated group, NR8383 stimulated with 1 μg/ml of LPS; emodin-treated group, NR8383 cel s pretreated with emodin for 30 mins, then stimulated with 1μg/ml of LPS. After 6 h of incubation, the cel s were harvested. Real time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-146a in the cel s, and the expression of tumor necrosis factor-α(TNF-α) and interleukin (IL-6) in NR8383 cel s were assayed by using Western blot. Results After stimulating NR8383 cel s with LPS, the expression of miR-146a and the level of TNF-αand IL-6 were al significantly highter than blank control group. Compared with LPS-stimulated group, the expression of miR-146a was significantly increased in emodin-treated group, but the level of TNF-α and IL-6 were down-regulated. Conclusion Emodin could up-regulated the expression of miR-146a after LPS-induced inflammation of NR8383 cel s, suggesting that miR-146a may be involved in regulation of rat alveolar macrophages inflammatory response.
