西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
11期
38-41
,共4页
王莲萍%刘专专%孙岩%杨玉华%孙裕
王蓮萍%劉專專%孫巖%楊玉華%孫裕
왕련평%류전전%손암%양옥화%손유
HPLC%生脉颗粒%五味子醇甲%五味子甲素%五味子乙素%含量测定%薄层鉴别
HPLC%生脈顆粒%五味子醇甲%五味子甲素%五味子乙素%含量測定%薄層鑒彆
HPLC%생맥과립%오미자순갑%오미자갑소%오미자을소%함량측정%박층감별
HPLC%ShengMai granules%schisandrin%deoxysachizandrin%schisandrin B%content determination%TLC
目的：建立生脉颗粒质量标准控制方法。方法：采用TLC五味子薄层鉴别方法以及HPLC法测定五味子中五味子醇甲的含量。色谱柱：phenomenexLuna C18(150×4.60 mm，5μm)；流动相：甲醇-0.1%磷酸溶液(60∶40)；流速：1.0 mL/min；检测波长：250 nm。结果：薄层鉴别方法简单，阴性无干扰；五味子醇甲在0.0712～2.136μg呈良好的线性关系，r=0.9999，平均回收率为97.23%；结论：该方法简单、快速、专属性强、可操作性强。
目的：建立生脈顆粒質量標準控製方法。方法：採用TLC五味子薄層鑒彆方法以及HPLC法測定五味子中五味子醇甲的含量。色譜柱：phenomenexLuna C18(150×4.60 mm，5μm)；流動相：甲醇-0.1%燐痠溶液(60∶40)；流速：1.0 mL/min；檢測波長：250 nm。結果：薄層鑒彆方法簡單，陰性無榦擾；五味子醇甲在0.0712～2.136μg呈良好的線性關繫，r=0.9999，平均迴收率為97.23%；結論：該方法簡單、快速、專屬性彊、可操作性彊。
목적：건립생맥과립질량표준공제방법。방법：채용TLC오미자박층감별방법이급HPLC법측정오미자중오미자순갑적함량。색보주：phenomenexLuna C18(150×4.60 mm，5μm)；류동상：갑순-0.1%린산용액(60∶40)；류속：1.0 mL/min；검측파장：250 nm。결과：박층감별방법간단，음성무간우；오미자순갑재0.0712～2.136μg정량호적선성관계，r=0.9999，평균회수솔위97.23%；결론：해방법간단、쾌속、전속성강、가조작성강。
Objective:To establish a method for quality standard of ShengMai granules. Methods:The contents of schisandrin in WuWeiZi (Schisandra chinensis) were determined by HPLC and distinguished by TLC. Chromatographic column:phenomenex Luna C18 (150×4.60 mm, 5μm);mobile phase:methyl alcohol-0.1%phosphoric acid solution (60∶40);flow rate:1.0 mL/min;detection wavelength:250 nm. Results:TLC method was simple and the blank test demonstrated no interference. Schisandrin demonstrated better linear relationship in the range between 0.0 712 and 2.136μg, r=0.9 99 9, average recovery rate was 97.23%. Conclusion:The method is simple, rapid, specific and feasible.
