西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
11期
32-33
,共2页
高效液相色谱法%制川乌%乌头碱%含量测定
高效液相色譜法%製川烏%烏頭堿%含量測定
고효액상색보법%제천오%오두감%함량측정
HPLC%processed ChuanWu%aconitine%content determination
目的：建立利用高效液相色谱法(HPLC)对中药饮片制川乌、乌头碱含量的检测方法。方法：利用高效液相色谱法对中药饮片制川乌、乌头碱的含量进行测定。结果：中药饮片中制川乌和乌头碱的线性范围分别为0.49～5.30μg(r=0.9999)、0.34～3.4μg(r=0.9999)，线性关系良好；制川乌的回收率为99.2%，乌头碱的回收率为98.6%。结论：该方法具有准确、高效、简便易行等特点，适用于同时测定中药饮片中制川乌和乌头碱的含量。
目的：建立利用高效液相色譜法(HPLC)對中藥飲片製川烏、烏頭堿含量的檢測方法。方法：利用高效液相色譜法對中藥飲片製川烏、烏頭堿的含量進行測定。結果：中藥飲片中製川烏和烏頭堿的線性範圍分彆為0.49～5.30μg(r=0.9999)、0.34～3.4μg(r=0.9999)，線性關繫良好；製川烏的迴收率為99.2%，烏頭堿的迴收率為98.6%。結論：該方法具有準確、高效、簡便易行等特點，適用于同時測定中藥飲片中製川烏和烏頭堿的含量。
목적：건립이용고효액상색보법(HPLC)대중약음편제천오、오두감함량적검측방법。방법：이용고효액상색보법대중약음편제천오、오두감적함량진행측정。결과：중약음편중제천오화오두감적선성범위분별위0.49～5.30μg(r=0.9999)、0.34～3.4μg(r=0.9999)，선성관계량호；제천오적회수솔위99.2%，오두감적회수솔위98.6%。결론：해방법구유준학、고효、간편역행등특점，괄용우동시측정중약음편중제천오화오두감적함량。
Objective:To establish the determination method for the contents of processed ChuanWu (Aconi-tum carmichaelii ) and aconitine by HPLC. Methods:The contents of processed ChuanWu and aconitine were detect-ed by HPLC. Results: Processed ChuanWu and aconitine showed better linear relationship in the ranges between 0.49 and 5.3 μg (r=0.999 9) and 0.34, 3.4μg (r=0.999 9);average recovery rates of processed ChuanWu and aconi-tine were 99.2%and 98.6%respectively. Conclusion:The method, accurate, efficient, simple and feasible, could be used for content determination of processed ChuanWu and aconitine simultaneously.
