CAJ | 학술논문

目的：建立虫创愈胶囊的质量标准。方法：采用薄层色谱法对制剂中红参、续断、骨碎补进行定性鉴别；采用高效液相色谱法对续断中川续断皂苷Ⅵ进行含量测定；色谱条件：C18柱(150 mm×4.6 mm，5μm)，流动相：乙腈-水(30∶70)。检测波长：212 nm。结果：在薄层色谱中可检出红参、续断、骨碎补的特征斑点，阴性对照无干扰；川续断皂苷Ⅵ在4.16～41.6 mg(r=0.9999)的范围内呈良好的线性关系，平均回收率为97.4%。结论：建立的虫创愈胶囊中主要活性成分的定性定量方法准确、专属性及重现性好，且快速简便，可作为该制剂质量的控制方法。
목적：건립충창유효낭적질량표준。방법：채용박층색보법대제제중홍삼、속단、골쇄보진행정성감별；채용고효액상색보법대속단중천속단조감Ⅵ진행함량측정；색보조건：C18주(150 mm×4.6 mm，5μm)，류동상：을정-수(30∶70)。검측파장：212 nm。결과：재박층색보중가검출홍삼、속단、골쇄보적특정반점，음성대조무간우；천속단조감Ⅵ재4.16～41.6 mg(r=0.9999)적범위내정량호적선성관계，평균회수솔위97.4%。결론：건립적충창유효낭중주요활성성분적정성정량방법준학、전속성급중현성호，차쾌속간편，가작위해제제질량적공제방법。
Objective: To establish quality standard of ZheChong ChuangYu capsules. Methods: HongShen (radix ginseng rubra), XuDuan (Radix dipsaci) and GuSuiBu (Davallia mariesii Moore ex Bak.) in the preparations were identified qualitatively by thin-layer chromatography (TLC);the contents of dipsacosideⅥwere determined by HPLC:chromatographic column:C18 column (150 mm×4.6 mm, 5μm), mobile phase:acetonitrile-water (30∶70). Detection wavelength:212nm. Results:Characteristic spots of HongShen, XuDuan and GuSuiBu were identified by TLC, and the blank test showed no interference;dipsacosideⅥshowed better linear relationship in the range be-tween 4.16 and 41.6 mg (r=0.999 9), average recovery rate was 97.4%. Conclusion:Quantitative and qualitative method for the determination of main active ingredients contained in ZheChong ChuangYu capsules is accurate, spe-cific, reproducible, rapid and convenient;it could be used for quality control of the preparation.