CAJ | 학술논문

【目的】研究新城疫病毒对鸡胚成纤维细胞中 TLRs 抗病毒信号通路和促炎症相关细胞因子的诱导作用。【方法】采用分离培养的原代鸡胚成纤维细胞感染中等毒力的新城疫病毒，并在感染后的4、8、12、16、24 h 收集细胞，利用定量 PCR 检测 TLRs 信号通路及促炎症相关细胞因子在细胞新城疫病毒感染过程中的表达变化。【结果】结果显示，TLR3、TLR7、TLR15，及其衔接蛋白基因 MYD88、TRIF ，以及下游基因 IFN-α、IFN-β、Mx 的表达量在病毒感染后呈现出显著上调（P ＜0．05）。同时促炎症细胞因子 IL-1β、IL-6、TNF-α的表达量在病毒感染后也表现出显著上调（P ＜0．05）。【结论】研究表明，在鸡胚成纤维细胞对新城疫病毒的识别过程中，由 TLR3、TLR7、TLR15介导抗病毒信号通路，同时诱导产生促炎症细胞因子 IL-1β、IL-6、TNF-α，并在新城疫病毒诱导的炎症反应过程中发挥重要的作用。
【목적】연구신성역병독대계배성섬유세포중 TLRs 항병독신호통로화촉염증상관세포인자적유도작용。【방법】채용분리배양적원대계배성섬유세포감염중등독력적신성역병독，병재감염후적4、8、12、16、24 h 수집세포，이용정량 PCR 검측 TLRs 신호통로급촉염증상관세포인자재세포신성역병독감염과정중적표체변화。【결과】결과현시，TLR3、TLR7、TLR15，급기함접단백기인 MYD88、TRIF ，이급하유기인 IFN-α、IFN-β、Mx 적표체량재병독감염후정현출현저상조（P ＜0．05）。동시촉염증세포인자 IL-1β、IL-6、TNF-α적표체량재병독감염후야표현출현저상조（P ＜0．05）。【결론】연구표명，재계배성섬유세포대신성역병독적식별과정중，유 TLR3、TLR7、TLR15개도항병독신호통로，동시유도산생촉염증세포인자 IL-1β、IL-6、TNF-α，병재신성역병독유도적염증반응과정중발휘중요적작용。
Objective]The purpose of this study is to investigate the induction of Toll-Like Recep-tors (TLRs)-mediated antiviral pathway and inflammatory cytokines in chicken embryo fibro-blasts (CEFs)infected with Newcastle disease virus.[Method]We isolated CEFs for infection with NDV,and then analyzed the expression levels of TLR-mediated antiviral pathway and in-flammatory cytokine genes in NDV-infected cell at 4,8,12,16,24 h.p.i (Hours-posted infec-tion)using qRT-PCR.[Results]Results showed that the expression of TLR3,TLR7,TLR15 were significantly induced in NDV-infected CEFs (P <0.05),and significant increase were also observed in downstream genes MYD88,TRIF ,IFN-α,IFN-β,Mx in NDV-infected chicken cells (P <0.05).In addition,we found that the expression levels of inflammatory cytokines,in-cluding IL-1β,IL-6,TNF-α,were also significantly up-regulated in NDV-infected chicken cell.[Conclusion]These results suggest that TLR3,TLR7,TLR15 play an important role in induc-tion of IL-6,IL-1β and TNF-α to defend against Newcastle disease virus infection in chicken cells.