CAJ | 학술논문

【目的】从烟草根际采集的土壤中分离筛选获得一株能产 IAA 的促生菌株 MY07，有助于研发微生物肥料，实现农业增产。【方法】通过对菌株 MY07进行形态特征、生理生化特征测定以及16S rDNA 序列分析对该菌进行鉴定，测定该菌发酵液产生 IAA 的量以优化其培养条件。【结果】鉴定结果表明该菌株为弯曲芽孢杆菌；确定培养时间为44 h、10％的装液量、温度为30℃和转速为150 r／min 的条件下最适合菌株 MY07产 IAA。【结论】菌株 MY07是可以作为功能微生物肥料研发的出发菌株。
【목적】종연초근제채집적토양중분리사선획득일주능산 IAA 적촉생균주 MY07，유조우연발미생물비료，실현농업증산。【방법】통과대균주 MY07진행형태특정、생리생화특정측정이급16S rDNA 서렬분석대해균진행감정，측정해균발효액산생 IAA 적량이우화기배양조건。【결과】감정결과표명해균주위만곡아포간균；학정배양시간위44 h、10％적장액량、온도위30℃화전속위150 r／min 적조건하최괄합균주 MY07산 IAA。【결론】균주 MY07시가이작위공능미생물비료연발적출발균주。
Objective]A PGPR MY07 was isolated from rhizosphere soil of tobacco.It was help-ful to further develop microbial fertilizers and to increase agricultural yield.[Method]Based on the morphological,physiological and phylogenetic analysis of 16S rDNA,MY07 was identified. The liquid fermentation conditions of MY07 was studied by measuring IAA content.[Results]MY07 was identified as Bacillus flexus .The best conditions on IAA biosynthesis by MY07 were as follows:44 h,30 ℃,medium volume 10% and shaking speed 150 r/min.[Conclusion]The i-solated strain MY07 is expected to be excellent microbial fertilizer.