四川农业大学学报
四川農業大學學報
사천농업대학학보
JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY
2014年
4期
398-407,425
,共11页
陶燕蓝%蓝增全%吴田
陶燕藍%藍增全%吳田
도연람%람증전%오전
葡萄柚%离体培养%根%不定芽
葡萄柚%離體培養%根%不定芽
포도유%리체배양%근%불정아
Citrus paradisi Macf.%culture in vitro%root%adventitious bud
【目的】建立葡萄柚(Citrus paradisi Macf.)离体再生体系,为后续遗传转化体系的建立奠定基础。【方法】以10个葡萄柚品种种子萌发的无菌苗根段为材料,在附加不同植物激素种类及浓度的培养基上进行离体培养,并获得再生植株。【结果】在 MS+0.5 mg/L 6-BA+0.5 mg/L NAA 培养基上培养可以诱导出愈伤组织,其中“帕利斯”的诱导率最高,达87.95%;在培养基 MS+2.0 mg/L 6-BA+0.5 mg/L NAA 可分化出丛生芽,其中“星路比”分化率最高为37.84%;分化出的丛生芽在 MS+2.0 mg/L 6-BA+0.2 mg/L NAA 培养基上可以进行增殖培养;在1/2 MS+1.0 mg/L IBA 上可诱导出不定根,形成完整再生植株。炼苗移栽成活率均可达75.47%~88.40%。【结论】通过对10个葡萄柚品种根段离体再生培养,建立了6个品种的离体再生体系。
【目的】建立葡萄柚(Citrus paradisi Macf.)離體再生體繫,為後續遺傳轉化體繫的建立奠定基礎。【方法】以10箇葡萄柚品種種子萌髮的無菌苗根段為材料,在附加不同植物激素種類及濃度的培養基上進行離體培養,併穫得再生植株。【結果】在 MS+0.5 mg/L 6-BA+0.5 mg/L NAA 培養基上培養可以誘導齣愈傷組織,其中“帕利斯”的誘導率最高,達87.95%;在培養基 MS+2.0 mg/L 6-BA+0.5 mg/L NAA 可分化齣叢生芽,其中“星路比”分化率最高為37.84%;分化齣的叢生芽在 MS+2.0 mg/L 6-BA+0.2 mg/L NAA 培養基上可以進行增殖培養;在1/2 MS+1.0 mg/L IBA 上可誘導齣不定根,形成完整再生植株。煉苗移栽成活率均可達75.47%~88.40%。【結論】通過對10箇葡萄柚品種根段離體再生培養,建立瞭6箇品種的離體再生體繫。
【목적】건립포도유(Citrus paradisi Macf.)리체재생체계,위후속유전전화체계적건립전정기출。【방법】이10개포도유품충충자맹발적무균묘근단위재료,재부가불동식물격소충류급농도적배양기상진행리체배양,병획득재생식주。【결과】재 MS+0.5 mg/L 6-BA+0.5 mg/L NAA 배양기상배양가이유도출유상조직,기중“파리사”적유도솔최고,체87.95%;재배양기 MS+2.0 mg/L 6-BA+0.5 mg/L NAA 가분화출총생아,기중“성로비”분화솔최고위37.84%;분화출적총생아재 MS+2.0 mg/L 6-BA+0.2 mg/L NAA 배양기상가이진행증식배양;재1/2 MS+1.0 mg/L IBA 상가유도출불정근,형성완정재생식주。련묘이재성활솔균가체75.47%~88.40%。【결론】통과대10개포도유품충근단리체재생배양,건립료6개품충적리체재생체계。
Objective]The objective of the study was to establish regeneration system of C.para-disi in vitro and to provide useful references for the application of genetic transformation tech-nique.[Method]The roots of aseptic seedlings germinated from the seeds of 10 grapefruit varie-ties were cultured in vitro to obtain regeneration plants in the medium with different hormones and concentrations.[Results]The callus were observed in the medium MS+0.5 mg/L 6-BA+0.5 mg/L NAA and Perlist had the highest callus induction frequency (87.95%).The differentia-tion of adventitious buds was observed in the medium MS+2.0 mg/L 6-BA+0.5 mg/L NAA and Star Ruby had the largest differentiation rate of adventitious buds (37.84%).The regeneration buds were multiplied in the medium MS+2.0 mg/L 6-BA+0.2 mg/L NAA.The adventitious roots were induced in the medium 1/2 MS +1.0 mg/L IBA and the regeneration plants finally were obtained.The survival rates of regenerative plant ranged from 75.47% to 88.40%.[Con-clusion]The regeneration systems of 6 grapefruit varieties were established based on the study.