CAJ | 학술논문

为得到大孔树脂纯化茄子皮红色素的最佳纯化条件，采用7种大孔吸附树脂对茄子皮红色素进行静态吸附和解吸实验，以筛选出纯化色素的最佳树脂．运用单因素和正交试验研究了茄子皮红色素在最佳树脂上的吸附和解吸工艺，经方差分析确定了吸附和解吸的最佳条件．最佳吸附条件为用p H为3的柠檬酸‐磷酸氢二钠缓冲液将色素粗提液稀释7倍（吸光度值0．533左右），吸附温度30℃，吸附时间90min；最佳解吸条件为乙醇浓度50％，解吸时间30min ，解吸温度30℃．选择L S A‐21大孔吸附树脂，通过动态试验，确定吸附速率为8B V／h ，洗脱速率为4B V／h ，未纯化前色价为28.9，经该工艺所得纯化物色价为126.0，提高到4.36倍．
위득도대공수지순화가자피홍색소적최가순화조건，채용7충대공흡부수지대가자피홍색소진행정태흡부화해흡실험，이사선출순화색소적최가수지．운용단인소화정교시험연구료가자피홍색소재최가수지상적흡부화해흡공예，경방차분석학정료흡부화해흡적최가조건．최가흡부조건위용p H위3적저몽산‐린산경이납완충액장색소조제액희석7배（흡광도치0．533좌우），흡부온도30℃，흡부시간90min；최가해흡조건위을순농도50％，해흡시간30min ，해흡온도30℃．선택L S A‐21대공흡부수지，통과동태시험，학정흡부속솔위8B V／h ，세탈속솔위4B V／h ，미순화전색개위28.9，경해공예소득순화물색개위126.0，제고도4.36배．
Seven kinds of macro‐porous resins for the isolation and purification of aubergine red pigment are determined .The single factor tests ,orthogonal experimental designs and variance analyses were ap‐plied to select the optimize manipulation parameters of the macro‐porous resin . T he results indicated that the optimal macro‐porous resin is LSA‐21 .T he optimal adsorption conditions are:the absorbance value of pigment solution 0 .533 ,temperature of 30℃ ,adsorption time of 90min .T he optimal desorption conditions are:ethanol of 50% ,desorption time of 30min ,temperature of 30℃ .The adsorption speed of 8BV/h and desorption speed of 4BV/h ,the purity of pigment is 126.0 ,4.36 times of the pre‐purifica‐tion .