CAJ | 학술논문

副溶血性弧菌（ Vibrio parahaemolyticus，Vp）是我国食源性疾病的首要致病因素，是海产品上市前的必检微生物．某种意义上讲，检测的增菌时间决定了海产品的质量安全性和市场价值．为缩短增菌时间，采用单因素试验和响应面分析法优化Vp培养条件和增菌培养基．单因素分析确定最适于Vp（ATCC 17802）生长的温度、氧浓度和培养方式分别为37℃、透气胶塞和摇床培养．以混合蛋白胨、牛肉膏、NaCl为主要影响因素，利用Design－Expert 8．0软件的Box－Behnken设计进行增菌培养基优化，得到的二次回归方程具有很高的拟合性，优化的培养基配方为：酪蛋白胨6．75 g／L、胰蛋白胨3．25 g／L、牛肉膏4．76 g／L、NaCl 36．75 g／L．利用优化培养基对模型进行验证，培养12 h的Vp菌体密度可达模型最大预测值的98．77％，说明获得的模型可以对ATCC17802的生长动态进行预测和分析．
부용혈성호균（ Vibrio parahaemolyticus，Vp）시아국식원성질병적수요치병인소，시해산품상시전적필검미생물．모충의의상강，검측적증균시간결정료해산품적질량안전성화시장개치．위축단증균시간，채용단인소시험화향응면분석법우화Vp배양조건화증균배양기．단인소분석학정최괄우Vp（ATCC 17802）생장적온도、양농도화배양방식분별위37℃、투기효새화요상배양．이혼합단백동、우육고、NaCl위주요영향인소，이용Design－Expert 8．0연건적Box－Behnken설계진행증균배양기우화，득도적이차회귀방정구유흔고적의합성，우화적배양기배방위：락단백동6．75 g／L、이단백동3．25 g／L、우육고4．76 g／L、NaCl 36．75 g／L．이용우화배양기대모형진행험증，배양12 h적Vp균체밀도가체모형최대예측치적98．77％，설명획득적모형가이대ATCC17802적생장동태진행예측화분석．
Vibrio parahaemolyticus is a primary causative factor of our country's reported foodborne diseases affecting consumers in food safety and the development of aquaculture seriously.In a sense, the enrichment time determined the shelf life of seafood, which can effect on the quality and market value of seafood.To reduce en-richment time, the single factor tests and response surface methodology was used to optimize culture conditions and enrichment medium of Vibrio parahaemolyticus.Through the single factor analysis, the optimum growth tem-perature, oxygen concentration and culture mode of Vibrio parahaemolyticus ATCC 17802 were 37°C, breathable rubber plug and shake cultivation respectively.The Vibrio parahaemolyticus enrichment medium was optimized using the Box -Behnken Design of Design -Expert 8.0 software with mixed peptone, beef extract and NaCl as the main influence factors.The results showed that the quadratic regression equation had a high fitting degree. The optimal medium formulations as follows:casein peptone 6.75 g/L, tryptone 3.25 g/L, beef paste 4.76 g/L, NaCl 36.75 g/L.The optimized medium was used to verify the regression model.Vibrio parahaemolyticus cell density OD600 value (0.4632) was equivalent to 98.77%of the maximum predicted value (0.46895) of the model after incubation for 12 h.These results indicated that this regression model can be used for prediction and analysis of ATCC17802 growth dynamics.