CAJ | 학술논문

研究了纳米Fe3 O4协同微生物降解黄褐土中的2，4，6-三氯联苯（PCB30），以PCB30为唯一碳源时降解菌的生长状况，以及微生物接菌量、纳米Fe3 O4投加量、PCB30浓度对黄褐土中PCB30降解的影响。PCBs降解菌经16S rDNA鉴定为假单胞菌Pseudomonas sp．，与草莓假单胞菌Pseudomonas sp．fragi同源性为75％。环境因素对黄褐土中PCB30降解效果有明显影响。微生物接菌量在0～0．8 mL（1×109 cfu·mL－1）、纳米Fe3 O4投加量在0～16．7 g·kg－1、PCB30浓度在0～10 mg·kg－1范围内时，PCB30残留率随着微生物接菌量、纳米Fe3 O4投加量以及PCB30浓度的增大而降低。当三者都分别达到各自范围的上限时，微生物单一体系、纳米Fe3 O4单一体系和纳米Fe3 O4／微生物协同体系中PCB30残留率在反应7 d后分别为63．18％、43．27％和26．28％；纳米Fe3 O4／微生物协同体系的降解效果明显优于微生物和纳米Fe3 O4单一体系。
연구료납미Fe3 O4협동미생물강해황갈토중적2，4，6-삼록련분（PCB30），이PCB30위유일탄원시강해균적생장상황，이급미생물접균량、납미Fe3 O4투가량、PCB30농도대황갈토중PCB30강해적영향。PCBs강해균경16S rDNA감정위가단포균Pseudomonas sp．，여초매가단포균Pseudomonas sp．fragi동원성위75％。배경인소대황갈토중PCB30강해효과유명현영향。미생물접균량재0～0．8 mL（1×109 cfu·mL－1）、납미Fe3 O4투가량재0～16．7 g·kg－1、PCB30농도재0～10 mg·kg－1범위내시，PCB30잔류솔수착미생물접균량、납미Fe3 O4투가량이급PCB30농도적증대이강저。당삼자도분별체도각자범위적상한시，미생물단일체계、납미Fe3 O4단일체계화납미Fe3 O4／미생물협동체계중PCB30잔류솔재반응7 d후분별위63．18％、43．27％화26．28％；납미Fe3 O4／미생물협동체계적강해효과명현우우미생물화납미Fe3 O4단일체계。
The degradation of 2,4,6-trichlorobiphenyl in alfisol by nanoscale Fe3 O4 combined with microorganism,the growth of cell with PCB30 as the sole carbon source,the effect of cells inoculation amount,nanoscale Fe3 O4 dosage and ini-tial PCB30 concentration were investigated.The PCBs degrader strain was identified as Pseudomonas sp.by 1 6S rDNA gene sequence phylogenetic analysis,and 75% homology with Pseudomonas sp.fragi.The degradation rates of PCB30 increased with the microbial inoculation amount increasing from 0~0.8 mL (1*1 09 cfu·mL-1 ),the nanoscale Fe3 O4 dosage from 0~1 6.7 g·kg-1 ,and the PCB30 initial concentration from 0~1 0 mg·kg-1 .When the three parameters reach the upper limit in the regions,the residual rates of PCB30 in the treatments of microorganism,nanoscale Fe3 O4 ,and nanoscale Fe3 O4/microorganism were 63.1 8%,43.27%and 26.28%,respectively.The degradation in the treatment of nanoscale Fe3 O4/mi-croorganism was much better than that in the treatment of nanoscale Fe3 O4 and microorganism.