CAJ | 학술논문

目的：研究北虫草小分子肽抗氧化和耐心肌缺氧的作用机制，为北虫草的进一步有效利用提出科学的解释和参考。方法：建立体外氧化化学模型和实验动物体内缺血模型，采用紫外分光光度计法测定小分子肽对羟基自由基（·OH-）、二苯代苦味肼基自由基（DPPH-·）和超氧阴离子自由基（O-2·）的清除能力；大鼠心肌耐缺氧实验采用器官阻断，心电监护仪观察法。结果：北虫草小分子肽和谷胱甘肽（GSH）对·OH-的清除率分别为（87.6±1.72）%和（70.4±0.94）%（P>0.05），对DPPH-·的清除率分别为（77.7±0.17）%和（55.7±0.29）%（P>0.05），对O-2·的清除率分别为（52.0±1.54）%和（87.6±1.47）%（P<0.05）；对照组和实验组大鼠的心肌耐缺氧时间分别为159.4±21.96 s和340.5±64.58 s（P<0.05），呈剂量-效应关系。结论：北虫草蛋白源小分子肽能够提高对·OH-和DPPH-·的清除率，延长心肌耐缺氧时间，具有抗氧化、耐缺氧的生物活性。
목적：연구북충초소분자태항양화화내심기결양적작용궤제，위북충초적진일보유효이용제출과학적해석화삼고。방법：건입체외양화화학모형화실험동물체내결혈모형，채용자외분광광도계법측정소분자태대간기자유기（·OH-）、이분대고미정기자유기（DPPH-·）화초양음리자자유기（O-2·）적청제능력；대서심기내결양실험채용기관조단，심전감호의관찰법。결과：북충초소분자태화곡광감태（GSH）대·OH-적청제솔분별위（87.6±1.72）%화（70.4±0.94）%（P>0.05），대DPPH-·적청제솔분별위（77.7±0.17）%화（55.7±0.29）%（P>0.05），대O-2·적청제솔분별위（52.0±1.54）%화（87.6±1.47）%（P<0.05）；대조조화실험조대서적심기내결양시간분별위159.4±21.96 s화340.5±64.58 s（P<0.05），정제량-효응관계。결론：북충초단백원소분자태능구제고대·OH-화DPPH-·적청제솔，연장심기내결양시간，구유항양화、내결양적생물활성。
Objective: To study the mechanism of antioxidant and hypoxia of north Cordyceps small molecule peptide. Methods: In order to establish in vitro oxidation chemistry model and experimental animals ischemia model, removal ability of hydroxyl radicals(·OH-), diphenyl bitter hydrazine free radical(DPPH-·) and superoxide anion free radical(O-2·) were determined by using ultraviolet spectrophotometer. Rat myocardial hypoxia experiment was did by using blocking organs and ECG monitor method. Results: Compared with glutathione(GSH), · OH-clearance rate for (87.6±1.72)% and (70.4±0.94)%, DPPH-· for (77.7±0.17)% and (55.7±0.29)%, O-2· for (52.0± 1.54)% and (87.6 ± 1.47)%(P<0.05), respertively. Compared with control group, the myocardial hypoxia time to 159.4 ± 21.96 s and 340.5 ± 64.58 s(P<0.05). A dose-effect relationship. Conclusion: The small molecular peptide come from north cordyceps protein source can improve the clearance rate of O-2· and DPPH-·, extend the time of myocardial hypoxia and showed the biological activity of antioxidant and hypoxia.