CAJ | 학술논문

目的：将不同质量浓度的卡那霉素、潮霉素加入楸树培养基中，研究卡那霉素、潮霉素对楸树组培苗生长的影响，以确定抗生素对楸树茎段分化与生根的敏感质量浓度。方法：待楸树继代、生根培养基灭菌后温度降至30~50℃，将不同质量浓度的卡那霉素、潮霉素经抽滤式灭菌加入培养基中，在培养基中接入楸树组培无菌茎段培养，观测茎段继代（增殖芽数、芽长、叶数等）、生根（发根数、根长、芽长等）生长指标并统计分析。结果：楸树组培继代培养基添加卡那霉素质量浓度为100 mg/L时组培瓶苗生长缓慢，浓度为150 mg/L时叶片大部分发白并干枯，茎段基部无愈伤组织形成，瓶苗基本停止生长，楸树继代瓶苗对卡那霉素耐受性范围为100~150 mg/L；添加潮霉素质量浓度为5 mg/L时瓶苗生长较为缓慢，浓度为10 mg/L时叶片开始干枯，茎段基部愈伤组织较小，瓶苗基本停止生长，楸树继代瓶苗对潮霉素耐受性范围为10 mg/L左右。楸树组培生根培养基添加卡那霉素质量浓度为100 mg/L时大部分茎段干枯，少部分为绿但未分化芽与根，浓度为150 mg/L时大部分茎段干枯，极少上部为绿，基部干枯，但未分化芽与根，楸树组培瓶苗生根培养苗对卡那霉素耐受性范围为100~150 mg/L；添加潮霉素质量浓度为5 mg/L时少部分茎段干枯，浓度为10 mg/L时大部分茎段干枯，少部分为绿，茎段未出现芽的分化与根的萌发现象，楸树组培瓶苗生根培养苗对潮霉素耐受性范围为5~10 mg/L。结论：卡那霉素、潮霉素对楸树组培苗生长有明显的抑制作用且与抗生素浓度呈负相关，但低质量浓度（1 mg/L）的潮霉素对楸树继代分化芽数有促进作用；同一抗生素对楸树不同无性系间组培苗生长的影响无显著差异。
목적：장불동질량농도적잡나매소、조매소가입추수배양기중，연구잡나매소、조매소대추수조배묘생장적영향，이학정항생소대추수경단분화여생근적민감질량농도。방법：대추수계대、생근배양기멸균후온도강지30~50℃，장불동질량농도적잡나매소、조매소경추려식멸균가입배양기중，재배양기중접입추수조배무균경단배양，관측경단계대（증식아수、아장、협수등）、생근（발근수、근장、아장등）생장지표병통계분석。결과：추수조배계대배양기첨가잡나매소질량농도위100 mg/L시조배병묘생장완만，농도위150 mg/L시협편대부분발백병간고，경단기부무유상조직형성，병묘기본정지생장，추수계대병묘대잡나매소내수성범위위100~150 mg/L；첨가조매소질량농도위5 mg/L시병묘생장교위완만，농도위10 mg/L시협편개시간고，경단기부유상조직교소，병묘기본정지생장，추수계대병묘대조매소내수성범위위10 mg/L좌우。추수조배생근배양기첨가잡나매소질량농도위100 mg/L시대부분경단간고，소부분위록단미분화아여근，농도위150 mg/L시대부분경단간고，겁소상부위록，기부간고，단미분화아여근，추수조배병묘생근배양묘대잡나매소내수성범위위100~150 mg/L；첨가조매소질량농도위5 mg/L시소부분경단간고，농도위10 mg/L시대부분경단간고，소부분위록，경단미출현아적분화여근적맹발현상，추수조배병묘생근배양묘대조매소내수성범위위5~10 mg/L。결론：잡나매소、조매소대추수조배묘생장유명현적억제작용차여항생소농도정부상관，단저질량농도（1 mg/L）적조매소대추수계대분화아수유촉진작용；동일항생소대추수불동무성계간조배묘생장적영향무현저차이。
Objective: To study the effect of kanamycin and hygromycin on the growth of tissue culture seedling of Catalpa bungei, the different concentrations of kanamycin and hygromycin were added into the subculture and rooting medium. We determined the concentration of antibiotic sensitivity of C.bungei subculture and rooting of stem segment. Methods: The different concentrations of kanamycin and hygromycin were added into the medium of the C.bungei subculture with filtration sterilization. The sterile stems were cultured in the medium, and the growth index such as bud length, bud number, leaf number etc. in subculture, and root number, root length, shoot length and so on in root were observed and statistical analyzed. Results: Experiments showed that C.bungei plant?let grew slowly when kanamycin concentration was 100 mg/L in subculture medium. When the kanamycin concen?tration was 150 mg/L, most of the leaves turned white and dried up, and the stem base was without callus, so tol?erance range of appropriate concentration to subculture was 100~150 mg/L. 5 mg/L hygromycin was added into subculture medium, C.bungei plantlet grew slowly. When the concentration reached 10 mg/L, the leaves dried up and stem base callus was smaller and plantlets stoped grow, thus tolerance range of hygromycin appropriate concen?tration to subculture was about 10 mg/L. Most C.bungei stem dried and a few green but not differentiation bud and root when 100 mg/L kanamycin was added into root medium. When kanamycin concentration was 150 mg/L, C. bungei stems were basic dead and no differentiation bud and root, and rooting seedlings on kanamycin tolerance range of 100~150 mg/L tissue culture plantlets of C.bungei. When hygromycin concentration was 5 mg/L, a few stem dried, at the concentration of 10 mg/L, most stem dried, some turned green, stems were without bud differen?tiation and root germination, seedlings of hygromycin tolerance range was 5~10 mg/L in rooting culture of C. bungei. Conclusion: Experiments showed that, kanamycin and hygromycin had a significant effect on the growth of C.bungei in vitro culture. Kanamycin and hygromycin inhibited and negatively related to the growth of tissue cul?tured seedlings of C.bungei, but low hygromycin concentration(1 mg/L) could promote differentiation buds in C. bungei subculture. The same antibiotics on different clones of C.bungei influence the growth of tissue culture seed?ling had no significant difference.