CAJ | 학술논문

目的：克隆一个水稻β-肌动蛋白基因，并研究其蛋白的亚细胞分布。方法：利用RT-PCR技术从日本晴水稻中扩增得到β-肌动蛋白基因OsActin，将其与拟南芥肌动蛋白基因家族进行分子进化分析；利用胶体金免疫电镜技术对水稻β-肌动蛋白基因进行定位分析。结果：核苷酸和氨基酸序列分析表明，OsActin与水稻的OsRAc1基因有较高的同源性（98%）；胶体金免疫电镜定位结果显示β-肌动蛋白在细胞核、细胞质中均有分布。结论：获得了水稻β-肌动蛋白基因OsActin，并研究了其蛋白在水稻韧皮部内的分布，为进一步探讨该基因的功能及作用机理奠定了基础。
목적：극륭일개수도β-기동단백기인，병연구기단백적아세포분포。방법：이용RT-PCR기술종일본청수도중확증득도β-기동단백기인OsActin，장기여의남개기동단백기인가족진행분자진화분석；이용효체금면역전경기술대수도β-기동단백기인진행정위분석。결과：핵감산화안기산서렬분석표명，OsActin여수도적OsRAc1기인유교고적동원성（98%）；효체금면역전경정위결과현시β-기동단백재세포핵、세포질중균유분포。결론：획득료수도β-기동단백기인OsActin，병연구료기단백재수도인피부내적분포，위진일보탐토해기인적공능급작용궤리전정료기출。
Objetive: To clone a rice β-actin gene and study the subcellular distribution of its protein. Meth?ods: A β-actin gene OsActin was amplified by RT-PCR from Nipponbare rice plant. Its molecular evolution was analyzed by comparing with the β-actin gene family from Arabidopsis thaliana and then its subcellular localization in rice was determined by colloidal gold immunoelectron microscopy technique. Results: Sequence analysis re?vealed that OsActin shared high homology(98%) with OsRAc1 gene at the nucleotide and amino acid levels. The results of immunogold electron microscopy showed that β-actin was distributed in both nucleus and cytoplasms of rice plant. Conclusion: The OsActin gene was obtained and the distribution of β-actin was studied in rice phlo?em. These results laid a foundation for further study on the function and mechanism of OsActin.