CAJ | 학술논문

目的：研究乳腺癌细胞中TANK结合激酶1（TBK1）的功能及分子机制。方法：利用含有雌激素应答元件（ERE）的萤光素酶报告基因检测TBK1对雌激素受体α（ERα）转录活性的影响；将TBK1参与激活先天免疫途径的关键位点突变，使其丧失激活先天免疫的功能，再用同样方法检测TBK1突变体对ERα转录活性的影响；采用RT-PCR检测TBK1通过磷酸化修饰对ERα下游基因表达水平的影响。结果：TBK1增强ERα的转录活性，从而增强其下游基因的表达。结论：TBK1能以不依赖于其激活先天免疫途径功能的方式增强ERα的转录活性。
목적：연구유선암세포중TANK결합격매1（TBK1）적공능급분자궤제。방법：이용함유자격소응답원건（ERE）적형광소매보고기인검측TBK1대자격소수체α（ERα）전록활성적영향；장TBK1삼여격활선천면역도경적관건위점돌변，사기상실격활선천면역적공능，재용동양방법검측TBK1돌변체대ERα전록활성적영향；채용RT-PCR검측TBK1통과린산화수식대ERα하유기인표체수평적영향。결과：TBK1증강ERα적전록활성，종이증강기하유기인적표체。결론：TBK1능이불의뢰우기격활선천면역도경공능적방식증강ERα적전록활성。
Objective: To explore the function of human TANK-binding kinase 1(TBK1) during the breast can?cer development. Methods: We used a luciferase reporter assay system to detect the transcriptional activity of es?trogen receptor α(ERα), the same system to test the function of TBK1 native-immunodeficiency mutant, and then RT-PCR method to test the effect of TBK1 on ERα target genes. Results: TBK1 increased ERα transcriptional ac?tivity and promoted expression of ERα-downstream genes. Conclusion: TBK1 increased ERα transcriptional activi?ty independent of its role in native immune.