CAJ | 학술논문

目的：在肝癌细胞系中过表达miR-155，研究其对肝癌细胞增殖的影响。方法：将pcDNA3.0-miR-155表达载体瞬时转染Huh7.5.1及Hcclm3肝癌细胞系，通过实时定量PCR技术对miR-155在转录水平的表达进行检测，采用CCK8法及克隆形成实验检测miR-155过表达后对Huh7.5.1及Hcclm3肝癌细胞系增殖的影响。结果：转染细胞后72 h，经实时定量PCR检测，Huh7.5.1及Hcclm3肝癌细胞中成熟miR-155的表达分别上调约431及16倍（P<0.01），说明其能有效高表达；CCK8法及克隆形成实验结果显示，miR-155能够明显促进肝癌细胞增殖（P<0.01）。结论：pcDNA3.0-miR-155转染Huh7.5.1及Hcclm3肝癌细胞系后能高效表达成熟miR-155，同时证明过表达miR-155能使肝癌细胞的增殖受到非常明显的促进。
목적：재간암세포계중과표체miR-155，연구기대간암세포증식적영향。방법：장pcDNA3.0-miR-155표체재체순시전염Huh7.5.1급Hcclm3간암세포계，통과실시정량PCR기술대miR-155재전록수평적표체진행검측，채용CCK8법급극륭형성실험검측miR-155과표체후대Huh7.5.1급Hcclm3간암세포계증식적영향。결과：전염세포후72 h，경실시정량PCR검측，Huh7.5.1급Hcclm3간암세포중성숙miR-155적표체분별상조약431급16배（P<0.01），설명기능유효고표체；CCK8법급극륭형성실험결과현시，miR-155능구명현촉진간암세포증식（P<0.01）。결론：pcDNA3.0-miR-155전염Huh7.5.1급Hcclm3간암세포계후능고효표체성숙miR-155，동시증명과표체miR-155능사간암세포적증식수도비상명현적촉진。
Objective: To study the effect of miR-155 overexpression on hepatocellular carcinoma cell prolifera?tion. Methods: pcDNA3.0-miR-155 was transiently transfected into Huh7.5.1 and Hcclm3 hepatocellular carcino?ma cell lines. RT-PCR assay was used to access the transcription level of miR-155. CCK8 assay and colony-form?ing unit assay were used to evaluate the effect of miR-155 overexpression on Huh7.5.1 and Hcclm3 cell prolifera?tion. Results: miR-155 could be highly efficient expressed 72 h post transfection, and the expression level was accordingly upregulated 431 and 16 folds respectively(P<0.01). CCK8 assay and colony-forming unit assay also showed that miR-155 could obviously stimulate hepatocellular carcinoma cell proliferation(P<0.01). Conclusion:miR-155 can be highly expressed in Huh7.5.1 and Hcclm3 hepatocellular carcinoma cell lines via pcDNA3.0-miR-155 transfection. Meanwhile, miR-155 overexpression can obviously promote hepatocellular carcinoma cell pro?liferation.