华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
6期
101-105
,共5页
谷静丛%王冲%王国琴%王鹏文
穀靜叢%王遲%王國琴%王鵬文
곡정총%왕충%왕국금%왕붕문
玉米自交系%SSR%杂交优势群
玉米自交繫%SSR%雜交優勢群
옥미자교계%SSR%잡교우세군
Maize inbred line%Simple sequence repeat%Heterotic group
为了明确110份玉米自交系的亲缘关系,更好地利用和改良玉米自交系,以代表国内主要杂种优势群的4个标准测验种(丹340、黄早4、Mo17、K12)与106份普通玉米自交系为材料,利用SSR分子标记进行遗传多样性分析,选用28对SSR引物对供试材料进行了遗传多样性研究。结果表明,在供试材料中共检测到125个等位基因变异,平均每对引物检测到的等位基因变异为4.46个,变异为2~10个,平均多态性信息量PIC值为0.85,变化范围为0.75~0.94。利用UPGMA聚类分析方法可将110份普通玉米自交系划分为6个类群。
為瞭明確110份玉米自交繫的親緣關繫,更好地利用和改良玉米自交繫,以代錶國內主要雜種優勢群的4箇標準測驗種(丹340、黃早4、Mo17、K12)與106份普通玉米自交繫為材料,利用SSR分子標記進行遺傳多樣性分析,選用28對SSR引物對供試材料進行瞭遺傳多樣性研究。結果錶明,在供試材料中共檢測到125箇等位基因變異,平均每對引物檢測到的等位基因變異為4.46箇,變異為2~10箇,平均多態性信息量PIC值為0.85,變化範圍為0.75~0.94。利用UPGMA聚類分析方法可將110份普通玉米自交繫劃分為6箇類群。
위료명학110빈옥미자교계적친연관계,경호지이용화개량옥미자교계,이대표국내주요잡충우세군적4개표준측험충(단340、황조4、Mo17、K12)여106빈보통옥미자교계위재료,이용SSR분자표기진행유전다양성분석,선용28대SSR인물대공시재료진행료유전다양성연구。결과표명,재공시재료중공검측도125개등위기인변이,평균매대인물검측도적등위기인변이위4.46개,변이위2~10개,평균다태성신식량PIC치위0.85,변화범위위0.75~0.94。이용UPGMA취류분석방법가장110빈보통옥미자교계화분위6개류군。
In order to specify the genetic relationship of 110 maize inbred lines,better use of and improve maize inbred lines,four key inbred lines representing 4 heterotic groups (Dan 340,Huangzao 4,Mo17,K12)were used dominantly in China and 106 ordinary maize inbred lines were selected for the genetic diversity analysis by SSR Markers 28 SSR primers were selected. The results showed that 125 alleles were detected in there inbred lines, bands,an average of 4. 46 alleles per primer,variation of 2-10 alleles for each primer,mean polymorphic informa-tion content of 0. 85 and its range from 0. 75 to 0. 94 were detected. The 110 inbred lines were divided into 6 groups by UPGMA method.