CAJ | 학술논문

为阐明GnRHR基因的多态性与崂山奶山羊产羔数的关系,设计7对引物,采用PCR-RFLP和PCR-SSCP技术检测GnRHR基因在不同产羔数崂山奶山羊的单核苷酸多态性,并分析其与产羔数的关系。结果显示：该基因存在2个多态位点,A261G突变对于初产母羊 GA型产羔数比 AA型多0.06只,比 GG型多0.04只,差异不显著( P>0．05)；对于经产母羊GA型产羔数比AA型多0.35只,差异显著(P<0.05),比GG型多0.45只,差异不显著(P>0．05)。 G55A位点共检测到GG和GA这2种基因型,其中对于初产母羊GG型比GA型产羔数多0.13只,经产母羊GG型比GA型产羔数多0.19只,但差异均不显著(P>0.05)。因此,GnRHR基因A261G突变位点可能是影响崂山奶山羊产羔性能一个潜在的有效分子标记。
위천명GnRHR기인적다태성여로산내산양산고수적관계,설계7대인물,채용PCR-RFLP화PCR-SSCP기술검측GnRHR기인재불동산고수로산내산양적단핵감산다태성,병분석기여산고수적관계。결과현시：해기인존재2개다태위점,A261G돌변대우초산모양 GA형산고수비 AA형다0.06지,비 GG형다0.04지,차이불현저( P>0．05)；대우경산모양GA형산고수비AA형다0.35지,차이현저(P<0.05),비GG형다0.45지,차이불현저(P>0．05)。 G55A위점공검측도GG화GA저2충기인형,기중대우초산모양GG형비GA형산고수다0.13지,경산모양GG형비GA형산고수다0.19지,단차이균불현저(P>0.05)。인차,GnRHR기인A261G돌변위점가능시영향로산내산양산고성능일개잠재적유효분자표기。
The objectives of the present study were to elucidate the polymorphism of GnRHR gene and its rela-tionship with litter size of Laoshan dairy goat. Seven pairs of primers were designed to detect single nucleotide poly-morphism ( SNP) of GnRHR gene in Laoshan dairy goat with different kids by PCR-RFLP and PCR-SSCP. The re-sults showed two mutations (A261G and G55A) were detected. For A261G locus in exon1,the does with genotype GA had 0. 06 and 0. 04 kids more than those with genotype AA and GG in primipara ewes,but the differences were not significant ( P>0 . 05 );the does with genotype GA had 0 . 35 and 0 . 45 kids more than those with genotype AA and GG in multiparity ewes (P>0. 05). For G55A locus,two genotypes (GG and GA) were detected,the does with genotype GG had 0. 13 and 0. 19 more than those with genotype GA in primipara ewes and multiparity ewes, respectively. However the differences were not significant (P >0. 05). These results preliminarily indicated that A261G locus of GnRHR gene is a potential molecular marker for improving litter size in dairy goats.