CAJ | 학술논문

为了探究AY 9944-A-7在3-异丁基-1-甲基黄嘌呤(3-isobutyl-1-methylxanthine,IBMX)阻滞的绵羊卵母细胞减数分裂恢复过程中的作用,将绵羊卵丘-卵母细胞复合体( COCs)、分离的卵丘-卵母细胞( split COCs)和脱卵丘后制成的裸卵(DOs)培养于含250μmol/L IBMX的体外成熟液中,分别添加10,20,30,40μmol/L的AY 9944-A-7以诱导卵母细胞恢复减数分裂。结果表明,10~40μmol/L 的 AY 9944-A-7极显著地提高了绵羊 COCs 的生发泡破裂(GVBD)率和第一极体(PBI)排出率(P<0.01)；添加AY 9944-A-7对IBMX阻滞的绵羊DOs 生发泡破裂和PBI排出没有显著的刺激作用(P>0.05)；40μmol/L AY 9944-A-7使绵羊COCs、split COCs、DOs的退化率极显著提高(P<0．01)。说明AY 9944-A-7能够诱导IBMX抑制的绵羊COCs恢复减数分裂成熟,但是过量的AY 9944-A-7对绵羊卵母细胞有毒性,AY 9944-A-7诱导绵羊COCs克服IBMX抑制恢复减数分裂成熟的适宜剂量为20μmol/L。
위료탐구AY 9944-A-7재3-이정기-1-갑기황표령(3-isobutyl-1-methylxanthine,IBMX)조체적면양란모세포감수분렬회복과정중적작용,장면양란구-란모세포복합체( COCs)、분리적란구-란모세포( split COCs)화탈란구후제성적라란(DOs)배양우함250μmol/L IBMX적체외성숙액중,분별첨가10,20,30,40μmol/L적AY 9944-A-7이유도란모세포회복감수분렬。결과표명,10~40μmol/L 적 AY 9944-A-7겁현저지제고료면양 COCs 적생발포파렬(GVBD)솔화제일겁체(PBI)배출솔(P<0.01)；첨가AY 9944-A-7대IBMX조체적면양DOs 생발포파렬화PBI배출몰유현저적자격작용(P>0.05)；40μmol/L AY 9944-A-7사면양COCs、split COCs、DOs적퇴화솔겁현저제고(P<0．01)。설명AY 9944-A-7능구유도IBMX억제적면양COCs회복감수분렬성숙,단시과량적AY 9944-A-7대면양란모세포유독성,AY 9944-A-7유도면양COCs극복IBMX억제회복감수분렬성숙적괄의제량위20μmol/L。
To evaluate the effects of AY 9944-A-7 on 3-isobutyl-1-methylxanthine ( IBMX )-arrested meiotic maturation in ovine oocytes,sheep cumulus oocyte complexes (COCs),split COCs and denuded oocytes (DOs) were cultured in medium supplemented with 250 μmol/L IBMX. Different concentrations of AY 9944-A-7 (0,10, 20,30,40 μmol/L) were added into maturation medium respectively to induce meiotic resumption of oocytes. The results showed that,AY 9944-A-7 supplementation in the range from 10 to 40 μmol/L increased the percentage of GVBD and the rate of PBI extrusion significantly in ovine COCs ( P<0 . 01 ) . While AY 9944-A-7 showed no signif-icantly stimulatory effect on the percentage of GVBD and the rate of PBI extrusion of ovine DOs arrested by IBMX (P>0. 05). And the highest dose tested (40 μmol/L) resulted in significant degenerations in ovine COCs,split COCs and DOs ( P<0 . 01 ) . The results indicated that AY 9944-A-7 induced meiotic resumption inhibited by IBMX in sheep COCs. But AY 9944-A-7 over dose showed toxic effects on ovine oocytes. The optimal dosage for AY 9944-A-7 to overcome the meiotic inhibition by IBMX and resume meiosis was 20 μmol/L.