CAJ | 학술논문

为探索持续NaCl胁迫对西洋菜叶绿素荧光参数与电子传递的影响,以西洋菜为材料,研究了不同浓度NaCl(1,2,3,4 g/kg)持续处理下幼苗生长、叶绿素含量及叶绿素荧光参数变化,结果表明,当NaCl浓度小于3 g/kg时,西洋菜随着盐处理浓度的增大,叶绿素含量升高,初始荧光( Fo)增大,最大荧光( Fm)、潜在光化学活性( Fv/Fo)、光系统Ⅱ(PSⅡ)最大光化学效率(Fv/Fm)减小,叶片转化光能的能力降低；实际量子产量Y(Ⅱ)下降,非光化学猝灭(NPQ)上升,叶片通过调节热耗散维持较高的光化光能量分配；光化学猝灭(qP)与PSⅡ电子传递速率(ETR)降低,质体醌( QA )趋于被还原状态,电子传递受到抑制。当NaCl浓度达4 g/kg 时,PSⅡ受损,QA过分还原,电子传递明显受到抑制,能量转化、传递与分配不能正常进行,根冠比较对照下降62.5%,植株长势明显减弱。由此说明,西洋菜对持续NaCl胁迫的耐受浓度是3 g/kg。
위탐색지속NaCl협박대서양채협록소형광삼수여전자전체적영향,이서양채위재료,연구료불동농도NaCl(1,2,3,4 g/kg)지속처리하유묘생장、협록소함량급협록소형광삼수변화,결과표명,당NaCl농도소우3 g/kg시,서양채수착염처리농도적증대,협록소함량승고,초시형광( Fo)증대,최대형광( Fm)、잠재광화학활성( Fv/Fo)、광계통Ⅱ(PSⅡ)최대광화학효솔(Fv/Fm)감소,협편전화광능적능력강저；실제양자산량Y(Ⅱ)하강,비광화학졸멸(NPQ)상승,협편통과조절열모산유지교고적광화광능량분배；광화학졸멸(qP)여PSⅡ전자전체속솔(ETR)강저,질체곤( QA )추우피환원상태,전자전체수도억제。당NaCl농도체4 g/kg 시,PSⅡ수손,QA과분환원,전자전체명현수도억제,능량전화、전체여분배불능정상진행,근관비교대조하강62.5%,식주장세명현감약。유차설명,서양채대지속NaCl협박적내수농도시3 g/kg。
Our objective was to explore the effects of continued NaCl Stress on chlorophyll fluorescence param-eters and electron transport of Nasturtium officinale seedlings. Nasturtium officinale seedlings were treated with con-tinued NaCl(0,1,2,3,4 g/kg)in plastic pots with soil. We studied the effects of continued NaCl on growth,chloro-phyll content and chlorophyll fluorescence parameters after 60 days. The results indicated that with the increase of NaCl concentration in the range of 1-3 g/kg,Chlorophyll content and minimal fluorescence ( Fo) increased,while maximum fluorescence ( Fm) ,potential photochemical efficiency ( Fv/Fo) ,photochemical maximum efficiency of PSⅡ( Fv/Fm) and the ability of leaves to transform energy decreased;Moreover,the actual quantum yield Y (Ⅱ) de-creased,while non-photochemical quenching coefficient ( NPQ ) increased, and leaves maintained a high actinic light energy distribution by adjusting the heat dissipation;In addition,photochemical quenching coefficient ( qP) and electron transport rate (ETR) decreased,plastoquinone (QA) tended to be restored state,and the electron transfer was inhibited. 4 g/kg NaCl treatment caused the damage of PSⅡ,meanwhile,QA was restored excessively, electron transfer was obviously inhibited,energy conversion,transmission and distribution could not work normally, root to shoot ratio was decreased by 62. 5% compared to the control,and the growth of Nasturtium officinale was weakened significantly. All results suggested that the tolerance concentration of continued NaCl stress on Nasturtium officinale R. Br. was 3 g/kg.