CAJ | 학술논문

为探讨TaAP1-3基因在小麦三雌蕊性状形成中的作用,从小麦三雌蕊近等基因系CM28和CM28TP中克隆得到3个同源基因TaAP1-3a、TaAP1-3b和TaAP1-3c。通过碱基序列、氨基酸序列、聚类及实时荧光定量分析表明,Ta-AP1-3a、TaAP1-3b和TaAP1-3c 基因cDNA 序列分别为1210,1208,1199 bp,ORF分别为825,816,855 bp,分别编码274,271,284个氨基酸残基。 TaAP1-3a、TaAP1-3b 和 TaAP1-3c 与中国春 TaAP1-3的核苷酸序列相似度分别为96．02%,93.1%,93.56%,氨基酸序列相似性高达99%,95%,97%,并且与 A 类基因 TaAGL29、OsMADS15、ZAP1、BM8、EnWM8、TaAP1-3聚集到 AP1/SUQA 亚家族 FUL2类群中。实时荧光定量分析表明, TaAP1-3a、TaAP1-3b 和TaAP1-3c在CM28和CM28TP的3个发育时期的小穗中均有表达,但各个时期的表达量有明显差异。 CM28中主要在二棱期-小花分化期表达,CM28TP中主要在雌雄蕊原基分化期表达。试验分析显示,TaAP1-3a、TaAP1-3b和TaAP1-3c可能具有上述A类基因相似的功能,其表达模式可能与小麦三粒/一粒性状形成相关。试验结果为进一步探讨该基因在小麦三雌蕊性状形成过程中的作用奠定了基础。
위탐토TaAP1-3기인재소맥삼자예성상형성중적작용,종소맥삼자예근등기인계CM28화CM28TP중극륭득도3개동원기인TaAP1-3a、TaAP1-3b화TaAP1-3c。통과감기서렬、안기산서렬、취류급실시형광정량분석표명,Ta-AP1-3a、TaAP1-3b화TaAP1-3c 기인cDNA 서렬분별위1210,1208,1199 bp,ORF분별위825,816,855 bp,분별편마274,271,284개안기산잔기。 TaAP1-3a、TaAP1-3b 화 TaAP1-3c 여중국춘 TaAP1-3적핵감산서렬상사도분별위96．02%,93.1%,93.56%,안기산서렬상사성고체99%,95%,97%,병차여 A 류기인 TaAGL29、OsMADS15、ZAP1、BM8、EnWM8、TaAP1-3취집도 AP1/SUQA 아가족 FUL2류군중。실시형광정량분석표명, TaAP1-3a、TaAP1-3b 화TaAP1-3c재CM28화CM28TP적3개발육시기적소수중균유표체,단각개시기적표체량유명현차이。 CM28중주요재이릉기-소화분화기표체,CM28TP중주요재자웅예원기분화기표체。시험분석현시,TaAP1-3a、TaAP1-3b화TaAP1-3c가능구유상술A류기인상사적공능,기표체모식가능여소맥삼립/일립성상형성상관。시험결과위진일보탐토해기인재소맥삼자예성상형성과정중적작용전정료기출。
To investigate the role of TaAP1-3 in the formation of wheat three pistils trait. Three homologous genes TaAP1-3a, TaAP1-3b and TaAP1-3c were cloned from wheat three pistils near-isogenic lines CM28 and CM28TP in this study. By nucleotide sequence,amino acid sequence,quantification and clustering analysis showed that the cloned cDNA of TaAP1-3a,TaAP1-3b and TaAP1-3c were 1 210,1 208,1 199 bp in length,respectively. TaAP1-3a,TaAP1-3b and TaAP1-3c genes respectively contained an 825,816,855 bp ORF which encode 274, 271,284 amino acids. The cDNA sequence of TaAP1-3a,TaAP1-3b and TaAP1-3c with TaAP1-3 were respectively 96. 02%,93. 1% and 93. 56% similarity. And the identities to TaAP1-3 in amino acids sequences were 99%, 95%,97% respectively. Cluster analysis showed that TaAP1-3a,TaAP1-3b and TaAP1-3c were clustered into FUL2 clade,and showed greater similarity to A class function genes, such as, TaAGL29, OsMADS15, ZAP1, BM8, En-WM8E and TaAP1-3 genes. Real-time analysis showed the expression level of TaAP1-3a,TaAP1-3b and TaAP1-3c in CM28 and CM28TP were significantly different. For CM28,high expression level was shared in two ridge until floret differentiation ( spikelet length 2-5 mm) . However,the expression level was higher in the pistil and stamen primordium (spikelets length 5-7mm) of CM28TP. Experimental analysis indicated that the genes of TaAP1-3a, TaAP1-3b,TaAP1-3c may have similar function with A class function genes of above-mentioned,and the difference of expression pattern in CM28 and CM28TP may be bound up with three-pistil traits. The results make the founda-tion to further explore their roles in forming three-pistil traits in wheat.