CAJ | 학술논문

为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期TuMV-C4抗性相关的主效QTL位点BrTuA09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个InDel在双亲间具有多态性,且条带单一、扩增稳定；连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTuA09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜TuMV抗病分子育种奠定了良好的基础。
위심조여TuMV항성기인긴밀련쇄적분자표기,선용고항병독병대백채자교계91-112화고감병독병자교계T12-19이급유이자위쌍친구건적포함100개주계적DH군체위재료,통과SSR화InDel표기적유전분석,재A09상정위료일개신적여대백채묘기TuMV-C4항성상관적주효QTL위점BrTuA09。재차기출상,침대해QTL위점소재적표기구간,근거작도군체쌍친적중측서결과,설계합성27대인물,기중11개InDel재쌍친간구유다태성,차조대단일、확증은정；련쇄분석발현,11개InDel표기균피정위재A09련쇄군상BrTuA09적치신구간。이용BC1군체진행표기험증발현,저사표기대고항단주선택적준학솔균체도78%이상,가응용우분자표기보조선택,위대백채TuMV항병분자육충전정료량호적기출。
Our research constructed a molecular genetic map with a line 91-112 ( highly resistant to TuMV-C4),a highly susceptible line T12-19 and the DH population derived from microspore culture of F1(91-112 × T12-19). A total of 45 markers were mapped to the A09 chromosome,and a new QTL-BrTuA09 controlling TuMV-C4 re-sistance was identified. On this basis,27 pairs of primers were designed by screening the InDel loci on A09 chromo-some of Chinese cabbage based on genome re-sequencing. The results showed that distinct PCR amplification prod-ucts were obtained from the InDel markers. Among these markers,11 InDel were polymorphic on the tested parent materials,and mapped to the A09 chromosome. By linkage analysis,these markers were tightly linked to the QTL-BrtuA09 . The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hy-brid breeding programs and improve the resistance to TuMV-C4 .