CAJ | 학술논문

目的：研究2型糖尿病患者肾脏不同病变时期血清 IL-17和 IL-22水平，并分析糖尿病肾病患者 IL-17和IL-22水平与肾功能关系。方法实验包括60名伴有或不伴有肾脏损伤的2型糖尿病患者和20名正常对照者（NC），根据尿白蛋白肌酐比值（ACR），糖尿病患者分三组：无蛋白尿组（NA，n＝22）、微量白蛋白尿组（MA，n＝18）和肾病组（DN，n＝20）。常规方法检测血清生化指标和尿蛋白含量，ELISA方法检测 IL-17和 IL-22水平。分析各组血清 IL-17和 IL-22水平差异，肾病组血清 IL-17、IL-22水平与肾功能血清学指标肌酐、尿素的关系。结果 DN组 IL-17和 IL-22水平显著高于 MA组、NA 组和CT 组（P＜0．05和P＜0．01），差异有统计学意义；MA、NA和CT三组之间 IL-17和 IL-22差异无统计学意义（P＞0．05）。肾病组血清 IL-17、IL-22水平与血清尿素、肌酐水平之间没有发现相关性（P＞0．05）。结论在2型糖尿病肾病中血清IL-17和IL-22水平升高，IL-17和IL-22可能对糖尿病肾病的致病起促进作用，但是它们并不能反映肾功能状态。
목적：연구2형당뇨병환자신장불동병변시기혈청 IL-17화 IL-22수평，병분석당뇨병신병환자 IL-17화IL-22수평여신공능관계。방법실험포괄60명반유혹불반유신장손상적2형당뇨병환자화20명정상대조자（NC），근거뇨백단백기항비치（ACR），당뇨병환자분삼조：무단백뇨조（NA，n＝22）、미량백단백뇨조（MA，n＝18）화신병조（DN，n＝20）。상규방법검측혈청생화지표화뇨단백함량，ELISA방법검측 IL-17화 IL-22수평。분석각조혈청 IL-17화 IL-22수평차이，신병조혈청 IL-17、IL-22수평여신공능혈청학지표기항、뇨소적관계。결과 DN조 IL-17화 IL-22수평현저고우 MA조、NA 조화CT 조（P＜0．05화P＜0．01），차이유통계학의의；MA、NA화CT삼조지간 IL-17화 IL-22차이무통계학의의（P＞0．05）。신병조혈청 IL-17、IL-22수평여혈청뇨소、기항수평지간몰유발현상관성（P＞0．05）。결론재2형당뇨병신병중혈청IL-17화IL-22수평승고，IL-17화IL-22가능대당뇨병신병적치병기촉진작용，단시타문병불능반영신공능상태。
Objective To investigate serum levels of IL-17 and IL-22 in type 2 diabetes patients with the different stages of kidney injury,and the relationship between levels of IL-17,IL-22 and renal function in patients with diabetic nephropathy.Methods Subjects included 60 type 2 diabetes patients with or without kidney injury and 20 normal con-trols (NC).Diabetes patients were divided into 3 groups by level of urinary albumin to creatinine ratio(ACR):no pro-teinuria group (NA,ACR<30 mg/g),microalbuminuria group (MA,30 mg/g>ACR>300 mg/g)and diabetic ne-phropathy group (DN,ACR>300 mg/g).Routine biochemical methods were applied to detect serum biochemistry markers and urine albumin levels,ELISA was used to detect IL-17 and IL-22 levels.Analysis differences of IL-17 and IL-22 among NA MA,DN and NC groups and correlation between levels of IL-17,IL-22 and urea,creatinine in DN group respectively.Results The levels of IL-17 and IL-22 were significantly higher in DN group than that in MA,NA and NC group (P<0.05 and P<0.01,respectively),However,there were not significant difference among MA,NA and NC group (P> 0.05).No correlations Lied between serum level of IL-17,IL-22 and urea,creatinine in DN group (P>0.05).Conclusion Levels IL-17 and IL-22 were increased in serum of type 2 diabetes patients with nephropathy. IL-17 and IL-22 may play role in the pathogenicity of diabetic nephropathy,but they don’t reflect the status of renal function.