CAJ | 학술논문

目的：检测乙肝肝硬化、原发性肝癌患者外周血调节性B细胞（Breg）的比率，了解 Breg与肝硬化、肝癌疾病之间的关系。方法以CD19＋CD5＋CD1d＋作为Breg标记，流式细胞术检测24例乙肝肝硬化患者、28例肝癌患者和20名健康对照者外周血Breg占B淋巴细胞的比率。结果肝硬化患者组Breg比率（29．90±9．19）％和肝癌患者组Breg比率（31．34±8．99）％均显著高于健康对照组（22．50±6．52）％，分别为P＝0．016和P＝0．003；肝硬化患者组和肝癌患者组间Breg比率无统计学意义（P＞0．05）。结论与对照组比较，Breg比率在肝硬化、肝癌患者外周血中显著升高，提示Breg细胞增加，与肝硬化、肝癌B细胞免疫调节紊乱相关。
목적：검측을간간경화、원발성간암환자외주혈조절성B세포（Breg）적비솔，료해 Breg여간경화、간암질병지간적관계。방법이CD19＋CD5＋CD1d＋작위Breg표기，류식세포술검측24례을간간경화환자、28례간암환자화20명건강대조자외주혈Breg점B림파세포적비솔。결과간경화환자조Breg비솔（29．90±9．19）％화간암환자조Breg비솔（31．34±8．99）％균현저고우건강대조조（22．50±6．52）％，분별위P＝0．016화P＝0．003；간경화환자조화간암환자조간Breg비솔무통계학의의（P＞0．05）。결론여대조조비교，Breg비솔재간경화、간암환자외주혈중현저승고，제시Breg세포증가，여간경화、간암B세포면역조절문란상관。
Objective To explore the relationship bewteen regulatory B cells (Breg)with liver cirrhosis and Hepatic carcinomaby analyzing the proportions of Bregs.Methods Taking CD1 9+CD5+CD1 d+ as the marker of the Breg,Flow cytometry was implemented to determine the proportions of Bregs among the B lymphocytes from the 24 patients with liver cirrhosis,28 ones with Hepatic carcinoma and 20 Healthy controls.Results The proportions of Bregs among pa-tients with liver cirrhosis and the ones with Hepatic carcinoma were significantly higher than those of the Healthy con-trols(29.90±9.19% vs 22.50±6.52%,P=0.016 vs P=0.003),and no significant difference was observed between the proportions among the patients with liver cirrhosis and with Hepatic carcinoma(P>0.05).Conclusion The pro-portions of Bregs among patients with liver cirrhosis and Hepatic carcinoma were obviously higher than that of healthy controls which indicates that the increase of Bregs may relate to the regulatory disorders of B lymphocytes in the pa-tients with liver cirrhosis and Hepatic carcinoma.