荧光PCR检测CYP2C19基因分型与氯吡格雷临床疗效的相关性研究
형광PCR검측CYP2C19기인분형여록필격뢰림상료효적상관성연구
Association between genetic polymorphisms of CYP2C19 and the clinical response to clopidogrel by fluorescence PCR
  • 万方数据
    中国实验诊断学 중국실험진단학
    CHINESE JOURNAL OF LABORATORY DIAGNOSIS
    2014年 12期 1948-1950 ,共3页

    金欣%陈水平%左向华%佟利威%袁方%许晓跃%吴海涵%李占甲%张伟%黄媛%尹秀云%于农

    금흔%진수평%좌향화%동리위%원방%허효약%오해함%리점갑%장위%황원%윤수운%우농

    CYP2C19%氯吡格雷%荧光PCR%ADP抑制率 CYP2C19%氯吡格雷%熒光PCR%ADP抑製率
    CYP2C19%록필격뢰%형광PCR%ADP억제솔
    CYP2C19%clopidogrel%fluorescence PCR%ADP inhibitory rate
    目的：通过分析CYP2C19基因型与 ADP抑制率之间的关系，探讨CYP2C19基因分型与氯吡格雷抗凝治疗效果的关系。方法选取急性冠脉综合征（ACS）并成功接受经皮冠状动脉介入治疗术（PCI）的患者55例，收集患者 EDTA抗凝静脉血提取血液基因组DNA，用实时荧光 PCR方法分析 CYP2C19基因型，测定每名患者 ADP抑制率，用统计学方法分析CYP2C19不同基因型患者 ADP抑制率的差异。结果所有入组患者根据基因型不同分为快代谢组占49．09％（27／55），中等代谢组占38．19％（21／55），慢代谢组占12．73％（7／55）。三组ADP抑制率比较，两两相比差异均有统计学意义（P＜0．01）。结论 CYP2C19基因多态性与氯吡格雷抵抗存在关联，快代谢型患者 PCI术后服用氯吡格雷疗效好于携带基因突变的患者，慢代谢型患者PCI术后服用氯吡格雷疗效差。
    목적：통과분석CYP2C19기인형여 ADP억제솔지간적관계，탐토CYP2C19기인분형여록필격뢰항응치료효과적관계。방법선취급성관맥종합정（ACS）병성공접수경피관상동맥개입치료술（PCI）적환자55례，수집환자 EDTA항응정맥혈제취혈액기인조DNA，용실시형광 PCR방법분석 CYP2C19기인형，측정매명환자 ADP억제솔，용통계학방법분석CYP2C19불동기인형환자 ADP억제솔적차이。결과소유입조환자근거기인형불동분위쾌대사조점49．09％（27／55），중등대사조점38．19％（21／55），만대사조점12．73％（7／55）。삼조ADP억제솔비교，량량상비차이균유통계학의의（P＜0．01）。결론 CYP2C19기인다태성여록필격뢰저항존재관련，쾌대사형환자 PCI술후복용록필격뢰료효호우휴대기인돌변적환자，만대사형환자PCI술후복용록필격뢰료효차。
    Objective To investigate the correlation the between genotypes of CYP2C19 and treatment effect of clo-pidogrel by analyzing the relationship between CYP2C19 and the ADP inhibition rates.Methods The EDTA anticoagu-lated venous blood of 5 5 post PCI patients who were diagnosed with acute coronary syndromes were collected,genomic DNAs were extracted and amplified by real-time fluorescent PCR,and then the ADP inhibition rates were detected.The data were analyzed by atatistical software.Results According to the different genotypes,all the patients were divided into three groups.Among the 55 patients,there were 49.09%(27/55)patients with extensive metabolizer group, 38.19% (21/55)patients with intermediate metabolizer group,12.73% (7/55)patients with poor metabolizer group. There were significant differences among the ADP inhibition rates of extensive metabolizer group,intermediate metabo-lizer group and poor metabolizer group.Conclusion There were association between CYP2C19 gene polymorphisms and clopidogrel resistance.The anticoagulant effect of clopidogrel in the CYP2C19 poor metabolism genotype patients was poor.
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