宜宾学院学报
宜賓學院學報
의빈학원학보
JOURNAL OF YIBIN UNIVERSITY
2014年
12期
118-120
,共3页
曾桢迦%李芳%贾黄%肖小君%傅慧
曾楨迦%李芳%賈黃%肖小君%傅慧
증정가%리방%가황%초소군%부혜
大花蕙兰%原球茎%增殖%优化
大花蕙蘭%原毬莖%增殖%優化
대화혜란%원구경%증식%우화
Cymbidium hybridum%protocorm%propagation%optimization
以大花蕙兰原球茎(PLB)为试验材料,比较不同浓度植物激素6-BA(6-苄氨基腺嘌呤)、NAA(萘乙酸),AC(活性炭),有机添加物(香蕉汁)及不同培养方式(固体培养、液体培养)等因子对大花蕙兰原球茎增殖效果的影响.结果表明:大花蕙兰PLB增殖以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+AC 0.1 mg/L结合液体振荡的培养方式效果较好,培养基中添加100 g/L香蕉汁对大花蕙兰PLB的增殖具有显著的促进作用.
以大花蕙蘭原毬莖(PLB)為試驗材料,比較不同濃度植物激素6-BA(6-芐氨基腺嘌呤)、NAA(萘乙痠),AC(活性炭),有機添加物(香蕉汁)及不同培養方式(固體培養、液體培養)等因子對大花蕙蘭原毬莖增殖效果的影響.結果錶明:大花蕙蘭PLB增殖以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+AC 0.1 mg/L結閤液體振盪的培養方式效果較好,培養基中添加100 g/L香蕉汁對大花蕙蘭PLB的增殖具有顯著的促進作用.
이대화혜란원구경(PLB)위시험재료,비교불동농도식물격소6-BA(6-변안기선표령)、NAA(내을산),AC(활성탄),유궤첨가물(향초즙)급불동배양방식(고체배양、액체배양)등인자대대화혜란원구경증식효과적영향.결과표명:대화혜란PLB증식이MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+AC 0.1 mg/L결합액체진탕적배양방식효과교호,배양기중첨가100 g/L향초즙대대화혜란PLB적증식구유현저적촉진작용.
The effects of different concentrations of plant hormones (6-BA、NAA), AC (activated carbon), organic com?pounds (banana juice) and different culture methods (solid culture and liquid culture) on the multiplication of PLB were studied by using Cymbidium hybridium PLB. The results indicated that the best method for PLB propagation was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+AC 0.1 mg/L associating with liquid shaking culture, adding banana juice 100 g/L in the medium could have significant role on the promotion of the multiplication of Cymbidium hybridium PLB.