CAJ | 학술논문

为了进一步提高猪瘟疫苗的免疫效果，探讨补中益气散对该疫苗的免疫增强作用，该研究以某猪场27头30日龄健康仔猪随机分为3组，对照组和试验1，2组．对照组仅注射猪瘟疫苗；试验1组用补中益气散拌料饲喂一周后注射疫苗；试验2组注射疫苗同时拌料饲喂补中益气散一周，中药用量均按0.2％加入饲料．分别于免疫当天、免疫接种后7，30 d无菌操作采集血样，运用正向间接血凝试验检测血清中的抗体水平．结果表明：补中益气散可显著提高血清中的抗体水平，免疫后30 d免疫保护率维持在88.9％以上，提示补中益气散具有显著增强猪瘟疫苗免疫效果的作用．
위료진일보제고저온역묘적면역효과，탐토보중익기산대해역묘적면역증강작용，해연구이모저장27두30일령건강자저수궤분위3조，대조조화시험1，2조．대조조부주사저온역묘；시험1조용보중익기산반료사위일주후주사역묘；시험2조주사역묘동시반료사위보중익기산일주，중약용량균안0.2％가입사료．분별우면역당천、면역접충후7，30 d무균조작채집혈양，운용정향간접혈응시험검측혈청중적항체수평．결과표명：보중익기산가현저제고혈청중적항체수평，면역후30 d면역보호솔유지재88.9％이상，제시보중익기산구유현저증강저온역묘면역효과적작용．
The experiment has been designed to investigate the effect of Buzhong Yiqi powder on Classical Swine Fever (SWF) vaccine in order to improve its immune effect .Classical Swine Fever ,which is mainly prevented and controlled by vaccine ,is one of the List A infectious diseases of animals .However ,the im‐mune effect with vaccine is not so good .The experiment has been designed to investigate the immune effect of Buzhong Yiqi powder on Classical Swine Fever vaccine before or after injecting .Twenty seven healthy 30‐day‐old piglets have been selected and divided into 3 groups :experimental group 1 and 2 ,and control group .For the control group ,only Classical Swine Fever vaccine is immunized .For the experimental group 1 ,vaccine is immunized after feeding Buzhong Yiqi powder with the proportion of 0 .2% in feeds for one week .But for the experimental group 2 ,vaccine is immunized ,and Buzhong Yiqi powder with the proportion of 0 .2% in feeds is fed at the same time .Serum antibody level is detected by the method of in‐direct hemagglutination test before and 7 ,30 d after immunization .The results show that serum antibody level is significantly increased after feeding Buzhong Yiqi powder . And the ratio of immunoprotection maintains at the level of 88 .9% .It suggests that Buzhong Yiqi powder can enhance the immune effect of Classical Swine Fever vaccine .