CAJ | 학술논문

目的：研究库存红细胞悬液在室温加温和恒温箱加温下红细胞携氧功能的变化。方法从4℃贮血冰箱中随机取出红细胞悬液40份，分别置于室温中（N 组）或37℃恒温培养箱（H组）加温30 min，分别取样测定电解质、血气分析、游离 Hb、ATP 含量、2，3-DPG 含量、氧亲和力（P50）。结果两组血 pH 值、血 Na＋都未有因加温而发生明显变化，血 K＋含量增加，游离 Hb 升高，葡萄糖下降但差异无统计学意义。加温后红细胞 ATP 含量、2，3-DPG 含量、P50增加（P ＜0.05）；与 N 组比较，H 组升高更明显（P＜0.05）。结论库血加温至37℃能够增加红细胞携氧功能，有助于提高红细胞输注的有效性。
목적：연구고존홍세포현액재실온가온화항온상가온하홍세포휴양공능적변화。방법종4℃저혈빙상중수궤취출홍세포현액40빈，분별치우실온중（N 조）혹37℃항온배양상（H조）가온30 min，분별취양측정전해질、혈기분석、유리 Hb、ATP 함량、2，3-DPG 함량、양친화력（P50）。결과량조혈 pH 치、혈 Na＋도미유인가온이발생명현변화，혈 K＋함량증가，유리 Hb 승고，포도당하강단차이무통계학의의。가온후홍세포 ATP 함량、2，3-DPG 함량、P50증가（P ＜0.05）；여 N 조비교，H 조승고경명현（P＜0.05）。결론고혈가온지37℃능구증가홍세포휴양공능，유조우제고홍세포수주적유효성。
Objective To study the change of the oxygen-carried function of stored red blood cells,which was warmed in room temperature and calorstat.Methods Randomly removed from 4 ℃storage refrigerator,red blood cell suspension were put in room temperature 30 min (group N)and 37℃ calorstat 30 min (group H),and samples taken to examine electrolyte,blood gas,free Hb,ATP content,2,3-DPG content,oxygen affinity(P50).Results In two groups,blood pH value and blood Na+ did not change obviously due to heating.The content of blood K+ and free Hb increased,glucose fell,but there was no statistically significant difference.The content of ATP and 2,3-DPG in eryth-rocyte increased and P50 increased after heating (P <0.05),which increased more significantly in group H compared with group N (P<0.05).Conclusion Heating stored blood up to 37 ℃ can in-crease the oxygen-carried function of red blood cells,which contribute to improve the effectiveness of transfusion.