CAJ | 학술논문

研究“肿瘤消”的抗肿瘤活性和作用，为临床用药提供依据。通过 MTT 法检测“肿瘤消”对多种肿瘤细胞株和正常细胞存活率的影响；采用 Hoechst 染色法和 DNA 片段化分析对“肿瘤消”诱导 MDCC-MSBl 细胞凋亡的作用进行检测；Western blot 检测“肿瘤消”诱导 MDCC-MSBl 细胞凋亡相关蛋白因子表达情况。MTT 法检测结果显示，“肿瘤消”对 MDCC-MSBl、C6、SP2／0和 A549细胞的增殖具有明显抑制作用，对293A 细胞增殖无显著影响；Hoechst 染色和 DNA 片段化分析均证实“肿瘤消”能诱导 MDCC-MSBl细胞凋亡；Western blot 检测发现“肿瘤消”诱导 MDCC-MSBl 细胞凋亡与凋亡因子 caspase 3、caspase 8和caspase 9的活化有关。证实“肿瘤消”具有诱导肿瘤细胞凋亡的作用。
연구“종류소”적항종류활성화작용，위림상용약제공의거。통과 MTT 법검측“종류소”대다충종류세포주화정상세포존활솔적영향；채용 Hoechst 염색법화 DNA 편단화분석대“종류소”유도 MDCC-MSBl 세포조망적작용진행검측；Western blot 검측“종류소”유도 MDCC-MSBl 세포조망상관단백인자표체정황。MTT 법검측결과현시，“종류소”대 MDCC-MSBl、C6、SP2／0화 A549세포적증식구유명현억제작용，대293A 세포증식무현저영향；Hoechst 염색화 DNA 편단화분석균증실“종류소”능유도 MDCC-MSBl세포조망；Western blot 검측발현“종류소”유도 MDCC-MSBl 세포조망여조망인자 caspase 3、caspase 8화caspase 9적활화유관。증실“종류소”구유유도종류세포조망적작용。
In order to study the anti-tumor effect and mechanism of “Zhong Liu Xiao”decoction.The anti-tumor effects of “Zhong Liu Xiao”decoctions were evaluated by the cell survival rates in variety tumor cell by MTT assay;The MDCC-MSBl cells apoptosis induced by “Zhong Liu Xiao”decoction was detected by Hoechst staining and DNA fragment analysis;The expression levels of apoptotic proteins in MDCC-MSBl cells induced by “Zhong Liu Xiao”decoctions were detected by Western blot.The results showed that“Zhong Liu Xiao”decoctions significantly inhibited the proliferation of MDCC-MSBl,C6,SP2/0 and A549 cells,but not normal 293A cells.“Zhong Liu Xiao”decoction could induce tumor cell apoptosis detected by hoechst staining and DNA fragment analysis.The apoptosis of MDCC-MSBl cells induced by “Zhong Liu Xiao”as related to the activation of caspase 3,caspase 8,caspase 9.In conclusion,“Zhong Liu Xiao”de-coction has a better effect for inducing apoptosis in tumor cells.