CAJ | 학술논문

采用 PCR 技术从致羔羊脑炎粪肠球菌 XJ05基因组中扩增得到 LuxS 基因的全序列，对其进行分析，同时与其他细菌相应基因序列及编码的氨基酸进行同源性分析；构建该基因的原核表达载体，对表达蛋白进行鉴定。结果显示，该粪肠球菌 XJ05的 LuxS 基因全长459 bp，编码152个氨基酸，具有功能序列HXXEH，核苷酸同源性与 GenBank 上的枯草芽胞杆菌和粪肠球菌 V583同源性最高，分别为61．2％和100％；构建的重组质粒经诱导后表达分子质量为20 ku 的蛋白质，Western blot 检测显示特异性条带。说明 XJ05的 luxS 基因与肠球菌 V583和枯草芽胞杆菌亲缘关系较近，表达的重组蛋白具有良好的反应原性。
채용 PCR 기술종치고양뇌염분장구균 XJ05기인조중확증득도 LuxS 기인적전서렬，대기진행분석，동시여기타세균상응기인서렬급편마적안기산진행동원성분석；구건해기인적원핵표체재체，대표체단백진행감정。결과현시，해분장구균 XJ05적 LuxS 기인전장459 bp，편마152개안기산，구유공능서렬HXXEH，핵감산동원성여 GenBank 상적고초아포간균화분장구균 V583동원성최고，분별위61．2％화100％；구건적중조질립경유도후표체분자질량위20 ku 적단백질，Western blot 검측현시특이성조대。설명 XJ05적 luxS 기인여장구균 V583화고초아포간균친연관계교근，표체적중조단백구유량호적반응원성。
LuxS gene was amplified from the chromosomal DNA of Enterococcus faecalis XJ05 inducing lamb encephalitis with PCR,the fragments were sequenced and analyzed and compared with the corre-sponding sequences in GenBank.The prokaryotic expression vector was constructed and the recombinant proteins were analyzed by SDS-PAGE and Western blot.The full-length gene was 459 bp,the higher ho-mologies were 61.2% and 100% compared with Bocillus cereus and Enterococcus faecalis V583.The gene encoded 152 amino acids and had a high conservation HXXEH and the expressed product was 20 ku,West-ern blot showed a specific band.The results indicated that LuxS gene of XJ05 has similar relationship with that of Bocillus cereus and Enterococcus faecalis V583,and the recombinant protein had good immunoge-nicity.